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dc.creatorGonçalves Machado, Larissa
dc.creatorPla Ferrer, Davinia
dc.creatorSanz, Libia
dc.creatorJorge, Roberta Jeane B.
dc.creatorLeitão De Araújo, Moema
dc.creatorAlves, Maria Lúcia M.
dc.creatorAlvares, Diego Janisch
dc.creatorDe Miranda, Joari
dc.creatorNowatzki, Jenifer
dc.creatorde Morais Zani, Karen
dc.creatorFernandes, Wilson
dc.creatorTanaka Azevedo, Anita Mitico
dc.creatorFernández Ulate, Julián
dc.creatorZingali, Russolina B.
dc.creatorGutiérrez, José María
dc.creatorCorrêa Netto, Carlos
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2017-07-03T16:15:12Z
dc.date.available2017-07-03T16:15:12Z
dc.date.issued2016-03-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1874391915002213
dc.identifier.issn1874-3919
dc.identifier.urihttps://hdl.handle.net/10669/30169
dc.description.abstractBothrops jararaca is a slender and semi-arboreal medically relevant pit viper species endemic to tropical and subtropical forests in southern Brazil, Paraguay, and northern Argentina (Misiones). Within its geographic range, it is often abundant and is an important cause of snakebite. Although no subspecies are currently recognized, geographic analyses have revealed the existence of two well-supported B. jararaca clades that diverged during the Pliocene ~ 3.8 Mya and currently display a southeastern (SE) and a southern (S) Atlantic rainforest (Mata Atlântica) distribution. The spectrum, geographic variability, and ontogenetic changes of the venom proteomes of snakes from these two B. jararaca phylogroups were investigated applying a combined venom gland transcriptomic and venomic analysis. Comparisons of the venom proteomes and transcriptomes of B. jararaca from the SE and S geographic regions revealed notable interpopulational variability that may be due to the different levels of population-specific transcriptional regulation, including, in the case of the southern population, a marked ontogenetic venom compositional change involving the upregulation of the myotoxic PLA2 homolog, bothropstoxin-I. This population-specific marker can be used to estimate the proportion of venom from the southern population present in the B. jararaca venom pool used for the Brazilian soro antibotrópico (SAB) antivenom production. On the other hand, the southeastern population-specific D49-PLA2 molecules, BinTX-I and BinTX-II, lend support to the notion that the mainland ancestor of Bothrops insularis was originated within the same population that gave rise to the current SE B. jararaca phylogroup, and that this insular species endemic to Queimada Grande Island (Brazil) expresses a pedomorphic venom phenotype. Mirroring their compositional divergence, the two geographic B. jararaca venom pools showed distinct bioactivity profiles. However, the SAB antivenom manufactured in Vital Brazil Institute neutralized the lethal effect of both venoms to a similar extent. In addition, immobilized SAB antivenom immunocaptured most of the venom components of the venoms of both B. jararaca populations, but did not show immunoreactivity against vasoactive peptides. The Costa Rican bothropic–crotalic–lachesic (BCL) antivenom showed the same lack of reactivity against vasoactive peptides but, in addition, was less efficient immunocapturing PI- and PIII-SVMPs from the SE venom, and bothropstoxin-I, a CRISP molecule, and a D49-PLA2 from the venom of the southern B. jararaca phylogroup. The remarkable paraspecificity exhibited by the Brazilian and the Costa Rican antivenoms indicates large immunoreactive epitope conservation across the natural history of Bothrops, a genus that has its roots in the middle Miocene. This article is part of a Special Issue entitled: Omics Evolutionary Ecologes_ES
dc.description.sponsorshipMinisterio de Ciencia é Innovación/[BFU2010-17373]//Españaes_ES
dc.description.sponsorshipMinisterio de Economía y Competitividad/[BFU2013-42833-P]//Españaes_ES
dc.description.sponsorshipPrograma iberoamericano de ciencia y tecnología para el Desarrollo/[BIOTOX P211RT0412]/CYTED/Españaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-652]/UCR/Costa Ricaes_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico/[Genoprot 560931/2010-7]/CNPq/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteomics; Volumen 135. 2016es_ES
dc.subjectVenom gland transcriptomicses_ES
dc.subjectAntivenomicses_ES
dc.subjectGeographic venom variationes_ES
dc.subjectBothrops jararacaes_ES
dc.subjectSnake venomes_ES
dc.subjectProteomicses_ES
dc.titleCombined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforestes_ES
dc.typeartículo científico
dc.identifier.doi10.1016/j.jprot.2015.04.029
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid25968638


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