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dc.creatorHerrera Vega, María
dc.creatorde Cássia de O. Collaço, Rita
dc.creatorVillalta Arrieta, Mauren
dc.creatorSegura Ruiz, Álvaro
dc.creatorVargas Arroyo, Mariángela
dc.creatorWright, Christine E.
dc.creatorPaiva, Owen K.
dc.creatorMatainaho, Teatulohi
dc.creatorJensen, Simon D.
dc.creatorLeón Montero, Guillermo
dc.creatorWilliams, David J.
dc.creatorRodrigues Simioni, Lea
dc.creatorGutiérrez, José María
dc.date.accessioned2017-07-03T19:54:19Z
dc.date.available2017-07-03T19:54:19Z
dc.date.issued2016-01-22
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0378427415301168es_ES
dc.identifier.issn0378-4274
dc.identifier.urihttps://hdl.handle.net/10669/30172
dc.description.abstractThe neuromuscular junction activity of Oxyuranus scutellatus venom and its presynaptic neurotoxin, taipoxin, and their neutralization by two antivenoms were examined in mouse phrenic nerve-diaphragm preparations. The action of taipoxin was also studied at 21 °C. The efficacy of the antivenoms was also assessed in an in vivo mouse model. Both antivenoms were effective in neutralizing the neuromuscular blocking activity in preincubation-type experiments. In experiments involving independent addition of venom and antivenoms, neutralization depended on the time interval between venom addition and antivenom application. When taipoxin was incubated for 5, 10 or 20 min at 21 °C, and antivenom added and temperature increased to 37 °C, neutralization was achieved only when the toxin was incubated for 5 or 10 min. The neutralization by the two antivenoms in an in vivo model showed that both whole IgG and F(ab′)2 antivenoms were effective in neutralizing lethality. Our findings highlight the very rapid action of taipan venom at the nerve terminal, and the poor capacity of antivenoms to revert neurotoxicity as the time interval between venom or taipoxin application and antivenom addition increased. Additionally the disparity between molecular masses of the active substances of the two antivenoms did not result in differences in neutralization.es_ES
dc.description.sponsorshipThe Australian National Health and Medical Research Council/[1033977]//Australiaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicology Letters; Volumen 241. 2016es_ES
dc.subjectTaipan venomes_ES
dc.subjectNeuromuscular blocking activityes_ES
dc.subjectPhrenic nerve-diaphragm preparationes_ES
dc.subjectNeutralizationes_ES
dc.subjectSnake antivenomes_ES
dc.titleNeutralization of the neuromuscular inhibition of venom and taipoxin from the taipan (Oxyuranus scutellatus) by F(ab0)2 and whole IgG antivenomses_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/j.toxlet.2015.11.020
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid26621539


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