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dc.creatorVásquez Mayorga, Marcela
dc.creatorFuchs Castillo, Eric J.
dc.creatorHernández Jiménez, Eduardo José
dc.creatorHerrera Murillo, Franklin
dc.creatorHernández López, Jesús
dc.creatorMoreira González, Ileana
dc.creatorArnáez Serrano, Elizabeth
dc.creatorBarboza Vargas, Natalia María
dc.date.accessioned2017-07-26T20:04:36Z
dc.date.available2017-07-26T20:04:36Z
dc.date.issued2017-02-09
dc.identifier.citationhttps://peerj.com/articles/2931/
dc.identifier.issn2167-8359
dc.identifier.otherPMC5345823
dc.identifier.urihttps://hdl.handle.net/10669/30396
dc.description.abstractWe estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He = 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC = 0.274) and inbreeding coefficient (f = − 0.102) were both low. Clustering was not related to the geographical origin of accessions. International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. Molecular markers identified only one non-toxic accession (JCCR-24) from Mexico. This work is part of a countrywide effort to characterize the genetic diversity of the Jatropha curcas germplasm bank in Costa Rica.es_ES
dc.description.sponsorshipConsejo Nacional de Rectores/[736-B1-660]/CONARE/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.rightsAtribución 3.0 Costa Rica*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/cr/*
dc.sourcePeerj; 2017es_ES
dc.subjectGenetic diversityes_ES
dc.subjectJatropha curcases_ES
dc.subjectSCARs markerses_ES
dc.subjectnrDNA-ITS regiones_ES
dc.subjectEST-SSRes_ES
dc.titleMolecular characterization and genetic diversity of Jatropha curcas L. in Costa Ricaes_ES
dc.typeartículo científico
dc.identifier.doi10.7717/peerj.2931
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.identifier.pmid28289556


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Atribución 3.0 Costa Rica
Except where otherwise noted, this item's license is described as Atribución 3.0 Costa Rica