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dc.creatorVásquez Mayorga, Marcela
dc.creatorFuchs Castillo, Eric J.
dc.creatorHernández Jiménez, Eduardo José
dc.creatorHerrera Murillo, Franklin
dc.creatorHernández López, Jesús
dc.creatorMoreira González, Ileana
dc.creatorArnáez Serrano, Elizabeth
dc.creatorBarboza Vargas, Natalia María
dc.description.abstractWe estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He = 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC = 0.274) and inbreeding coefficient (f = − 0.102) were both low. Clustering was not related to the geographical origin of accessions. International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. Molecular markers identified only one non-toxic accession (JCCR-24) from Mexico. This work is part of a countrywide effort to characterize the genetic diversity of the Jatropha curcas germplasm bank in Costa Rica.es_ES
dc.description.sponsorshipConsejo Nacional de Rectores/[736-B1-660]/CONARE/Costa Ricaes_ES
dc.rightsAtribución 3.0 Costa Rica*
dc.sourcePeerj; 2017es_ES
dc.subjectGenetic diversityes_ES
dc.subjectJatropha curcases_ES
dc.subjectSCARs markerses_ES
dc.subjectnrDNA-ITS regiones_ES
dc.titleMolecular characterization and genetic diversity of Jatropha curcas L. in Costa Ricaes_ES
dc.typeartículo científico
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES

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Atribución 3.0 Costa Rica
Except where otherwise noted, this item's license is described as Atribución 3.0 Costa Rica