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Production and partial characterization of monoclonal antibodies to Bothrops asper (terciopelo) myotoxin
dc.creator | Lomonte, Bruno | |
dc.creator | Kahan, Lawrence | |
dc.date.accessioned | 2017-08-08T21:51:47Z | |
dc.date.available | 2017-08-08T21:51:47Z | |
dc.date.issued | 1988 | |
dc.identifier.citation | http://www.sciencedirect.com/science/article/pii/0041010188902498 | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/72949 | |
dc.description.abstract | Seven murine monoclonal antibodies against Bothrops aspen myotoxin were produced and partially characterized. They revealed the presence of at least four cross-reacting basic components in crude venom, with a common subunit mol. wt of 16,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis, but slight differences in charge. These myotoxin-related components might be isoforms of the toxin . By Western blotting and enzyme-immunoassay binding techniques, differences in the reactivities with basic venom fractions were observed among monoclonal antibodies, suggesting differences in epitope specifidties . Three antibodies cross-reacted with B. nummifer crude venom. Two monoclonal antibodies were utilized to develop a two-site enzyme-immunoassay for myotoxin detection at the nanogram level . Three of the antibodies (one IgM and two IgG,) showed ability to neutralize myotoxiclty of purified myotoxin in preincubation-type experiments. | es_ES |
dc.description.sponsorship | University of Wisconsin/[08387]//Estados Unidos | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon 26(7), 675-689 (1988) | es_ES |
dc.subject | anticuerpos | es_ES |
dc.subject | monoclonales | es_ES |
dc.subject | miotoxina | es_ES |
dc.title | Production and partial characterization of monoclonal antibodies to Bothrops asper (terciopelo) myotoxin | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/0041-0101(88)90249-8 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.pmid | 3140427 |
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Microbiología [1171]