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A high performance liquid chromatography method with electrochemical detection of gamma-aminobutyric acid, glutamate and glutamine in rat brain homogenates
dc.creator | Monge Acuña, Andrea A. | |
dc.creator | Fornaguera Trías, Jaime | |
dc.date.accessioned | 2017-12-18T13:34:45Z | |
dc.date.available | 2017-12-18T13:34:45Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | http://www.sciencedirect.com/science/article/pii/S0165027009003501?via%3Dihub | |
dc.identifier.issn | 0165-0270 | |
dc.identifier.uri | https://hdl.handle.net/10669/73691 | |
dc.description.abstract | Determination of γ-aminobutyric acid (GABA), glutamate (Glu) and glutamine (Gln) in animal models has been important to understand the normal function and clinical aspects of some neurological diseases. Quantification of these amino acid transmitters has conventionally been performed by using a high performance liquid chromatography (HPLC) system. This paper describes an improved HPLC method with electrochemical detection for glutamate, glutamine and GABA determination in brain homogenates. The protocol is based on a precolumn derivatization of amino acids with o-phthalaldehyde and sodium sulfite, a separation through a C18, 5 μm particle size column and an isocratic elution. Several modifications of previous works on methanol percentage, pH, temperature, flow rate and derivatization solution concentration were done to obtain a suitable protocol for amino acid quantification in brain homogenate samples. Total elution time is 35 min approximately. Technical requirements and laboratory expenses of this new protocol are minimal. This technique showed high linearity, repeatability and accuracy. | es_ES |
dc.description.sponsorship | Vicerrectoria de Investigación, Universidad de Costa Rica/[422-A5 524]/VINV/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Journal of Neuroscience Method, Vol. 183 (2), pp. 176–181 | es_ES |
dc.subject | GABA | es_ES |
dc.subject | Glutamate | es_ES |
dc.subject | Glutamine | es_ES |
dc.subject | HPLC | es_ES |
dc.subject | Isocratic elution | es_ES |
dc.subject | Neurotransmitters | es_ES |
dc.subject | Hippocampus | es_ES |
dc.subject | Rats | es_ES |
dc.title | A high performance liquid chromatography method with electrochemical detection of gamma-aminobutyric acid, glutamate and glutamine in rat brain homogenates | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/j.jneumeth.2009.06.042 | |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicina | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Neurociencias (CIN) | |
dc.identifier.pmid | 19596377 |