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dc.creatorPark, Sung Ryeol
dc.creatorTripathi, Ashootosh
dc.creatorWu, Jianfeng
dc.creatorSchultz, Pamela J.
dc.creatorYim, Isaiah
dc.creatorMcQuade, Thomas J.
dc.creatorYu, Fengan
dc.creatorArevang, Carl Johan
dc.creatorMensah, Abraham Y.
dc.creatorTamayo Castillo, Giselle
dc.creatorXi, Chuanwu
dc.creatorSherman, David H.
dc.identifier.otherArticle number: 10710 (2016)
dc.description.abstractPathogenic microorganisms often have the ability to attach to a surface, building a complex matrix where they colonize to form a biofilm. This cellular superstructure can display increased resistance to antibiotics and cause serious, persistent health problems in humans. Here we describe a high-throughput in vitro screen to identify inhibitors of Acinetobacter baumannii biofilms using a library of natural product extracts derived from marine microbes. Analysis of extracts derived from Streptomyces gandocaensis results in the discovery of three peptidic metabolites (cahuitamycins A–C), with cahuitamycin C being the most effective inhibitor (IC50=14.5 μM). Biosynthesis of cahuitamycin C proceeds via a convergent biosynthetic pathway, with one of the steps apparently being catalysed by an unlinked gene encoding a 6-methylsalicylate synthase. Efforts to assess starter unit diversification through selective mutasynthesis lead to production of unnatural analogues cahuitamycins D and E of increased potency (IC50=8.4 and 10.5 μM).es_ES
dc.description.sponsorshipGreat Lakes Regional Center of Excellence for Biodefense and Emerging Infectious Diseases/[U54 AI57153]/GLRCE/Estados Unidoses_ES
dc.description.sponsorshipArmy Research Office/[W911NF-12-1-0059]/ARO/Estados Unidoses_ES
dc.description.sponsorshipNational Institutes of Health/[1R01GM098350]/NIH/Estados Unidoses_ES
dc.description.sponsorshipInternational Cooperative Biodiversity Groups-Fogarty International Center/[U01 TW007404]/ICBG/Estados Unidoses_ES
dc.rightsAtribución 4.0 Internacional*
dc.sourceNature Communications, Vol. 7, 10710, 2016es_ES
dc.subjectNatural productses_ES
dc.titleDiscovery of cahuitamycins as biofilm inhibitors derived from a convergent biosynthetic pathwayes_ES
dc.typeartículo original
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES

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Atribución 4.0 Internacional
Except where otherwise noted, this item's license is described as Atribución 4.0 Internacional