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dc.creatorAguilar Alvarez, Estela Yamileth
dc.creatorRodríguez Solís, Carlos Mario
dc.creatorGarita Salazar, Laura Cristina
dc.creatorVillalobos Muller, William
dc.creatorMoreira Carmona, Lisela
dc.creatorHartung, John
dc.creatorRivera Herrero, Carmen
dc.creatorMontero Astúa, Mauricio
dc.creatorChacón Díaz, Carlos
dc.descriptionCopyright The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2008. / . Datos incluidos por Lisela Moreira Carmona.es_ES
dc.description.abstractCoffee plants exhibiting a range of symptoms including mild to severe curling of leaf margins, chlorosis and deformation of leaves, stunting of plants, shortening of internodes, and dieback of branches have been reported since 1995 in several regions of Costa Rica's Central Valley. The symptoms are referred to by coffee producers in Costa Rica as "crespera" disease and have been associated with the presence of the bacterium Xylella fastidiosa. Coffee plants determined to be infected by the bacterium by enzyme linked immunosorbent assay (ELISA), were used for both transmission electron microscopy (TEM) and for isolation of the bacterium in PW broth or agar. Petioles examined by TEM contained rod-shaped bacteria inside the xylem vessels. The bacteria measured 0.3 to 0.5 μm in width and 1.5 to 3.0 μm in length, and had rippled cell walls 10 to 40 nm in thickness, typical of X. fastidiosa. Small, circular, dome-shaped colonies were observed 7 to 26 days after plating of plant extracts on PW agar. The colonies were comprised of Gram-negative rods of variable length and a characteristic slight longitudinal bending. TEM of the isolated bacteria showed characteristic rippled cell walls, similar to those observed in plant tissue. ELISA and PCR with specific primer pairs 272-l-int/272-2-int and RST31/RST33 confirmed the identity of the isolated bacteria as X. fastidiosa. RFLP analysis of the amplification products revealed diversity within X. fastidiosa strains from Costa Rica and suggest closer genetic proximity to strains from the United States of America than to other coffee or citrus strains from Brazil.es_ES
dc.description.sponsorshipFundación CR-USAes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[801-A2-528]/UCR/Costa Ricaes_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.sourceThe Journal of Microbiology, vol.46(5), pp.482-490.es_ES
dc.subjectPierce’s diseasees_ES
dc.subjectCitrus variegated chlorosises_ES
dc.subjectCoffee leaf scorches_ES
dc.subject633.737 286 Cafées_ES
dc.titleIsolation and molecular characterization of Xylella fastidiosa from coffee plants in Costa Rica.es_ES
dc.typeartículo científicoes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional