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Modified Laccase-Gold Nanoparticles-Tetrathiafulvalene-SPCEs Based Biosensor to Determine W(VI) in Water

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Date
2017
Author
Alvarado Gámez, Ana Lorena
Arcos Martínez, María Julia
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Abstract
It was developed an amperometric biosensor to determine tungsten in water, based on the inhibition of laccase enzyme, by tungsten ions using pyrocathecol as a substrate. The enzyme was immobilized with a proper mixture containing, bovine serum albumin, and glutaraldehyde, for a cross-linking process over screen-printed carbon electrodes, previously modified with tetrathiafulvalen and gold nanoparticles. Optimized experimental conditions are: pyrocatechol in cell 0.040 mM in a phosphate buffer pH 6.5 and applied potential +350 mV. The repeatability and reproducibility, in terms of relative standard deviation values, of de developed biosensor were 3.3 % (n=3), and 2.2 % (n = 5) respectively, and detection limit was 1.8 × 10-7 mol L-1. Additionally it was determined the kinetics of the systems by means of Michaelis-Menten Km apparent constants, calculated using Lineweaver-Burk plots, with and without tungsten. Kinetic study resembles to be competitive inhibition. A recovery study was performed with spiked blanks with a tungsten certified reference standard, traceable to NIST, giving as a result 102.3 ± 6.7 %; tap water samples analyzed presented a mean concentration of 1.75 µM, and recovery of the tungsten certified reference standard on the tap water samples gave 98.8 ± 3.1 %.
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https://hdl.handle.net/10669/80661
https://www.sensorsportal.com/HTML/DIGEST/P_2915.htm
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  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
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  • Biblioteca Digital Carlos Melendez (CIHAC)
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Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.