dc.creator | Alvarado Gámez, Ana Lorena | |
dc.creator | Arcos Martínez, María Julia | |
dc.date.accessioned | 2020-02-28T15:38:09Z | |
dc.date.available | 2020-02-28T15:38:09Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | https://www.sensorsportal.com/HTML/DIGEST/P_2915.htm | |
dc.identifier.issn | 2306-8515 | |
dc.identifier.issn | 1726-5479 | |
dc.identifier.uri | https://hdl.handle.net/10669/80661 | |
dc.description.abstract | It was developed an amperometric biosensor to determine tungsten in water, based on the inhibition of
laccase enzyme, by tungsten ions using pyrocathecol as a substrate. The enzyme was immobilized with a proper
mixture containing, bovine serum albumin, and glutaraldehyde, for a cross-linking process over screen-printed
carbon electrodes, previously modified with tetrathiafulvalen and gold nanoparticles. Optimized experimental
conditions are: pyrocatechol in cell 0.040 mM in a phosphate buffer pH 6.5 and applied potential +350 mV. The
repeatability and reproducibility, in terms of relative standard deviation values, of de developed biosensor were
3.3 % (n=3), and 2.2 % (n = 5) respectively, and detection limit was 1.8 × 10-7 mol L-1. Additionally it was
determined the kinetics of the systems by means of Michaelis-Menten Km apparent constants, calculated using
Lineweaver-Burk plots, with and without tungsten. Kinetic study resembles to be competitive inhibition. A
recovery study was performed with spiked blanks with a tungsten certified reference standard, traceable to NIST,
giving as a result 102.3 ± 6.7 %; tap water samples analyzed presented a mean concentration of 1.75 µM, and
recovery of the tungsten certified reference standard on the tap water samples gave 98.8 ± 3.1 %. | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[804-B5-164]/UCR/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Sensors & Transducers, vol. 211(4), pp.1-7 | es_ES |
dc.subject | Biosensor | es_ES |
dc.subject | Cathecol | es_ES |
dc.subject | Gold nanoparticles | es_ES |
dc.subject | Laccase | es_ES |
dc.subject | Screen printed carbon electrodes | es_ES |
dc.subject | Tetrathiafulvalen | es_ES |
dc.subject | Tungsten | es_ES |
dc.subject | Water | es_ES |
dc.title | Modified Laccase-Gold Nanoparticles-Tetrathiafulvalene-SPCEs Based Biosensor to Determine W(VI) in Water | es_ES |
dc.type | artículo original | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ) | es_ES |
dc.identifier.codproyecto | 804-B5-164 | |