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dc.creatorCastro Gutiérrez, Víctor Manuel
dc.creatorHassard, Francis
dc.creatorMoir, James W.B.
dc.date.accessioned2022-06-23T21:15:51Z
dc.date.available2022-06-23T21:15:51Z
dc.date.issued2022-04
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0167701222000422es_ES
dc.identifier.issn0167-7012
dc.identifier.urihttps://hdl.handle.net/10669/86777
dc.description.abstractMetaldehyde, a molluscicide pesticide, has been identified as a pollutant of concern due to its repeated detection in drinking water, thereby generating numerous compliance failures for water utilities. Biological degradation potential for metaldehyde is widespread in soils, occurring at different rates, but to date, no molecular methods for its assessment have been reported. Here, three genes belonging to a shared metaldehyde-degrading gene cluster present in bacteria were used as candidates for development of a quantitative PCR (qPCR) assay for assessing the metaldehyde-degrading potential in soil. Screening of gene targets, primer pairs and optimization of reaction conditions led to the development of a sensitive and specific probe-based qPCR method for quantifying the mahY metaldehyde-degrading gene from soil. The technique was tested across 8 soils with different compositions and origins. The degrading pathway was detected in 4/8 soils, in which a higher number of gene copies correlated with periods of greater metaldehyde removal. Additionally, swift elimination of the pesticide was observed in soils with an elevated initial number of mahY gene copies. The gene cluster was not detected in other soils, even though metaldehyde removal occurred, indicating that other biological degrading pathways are also important in nature. The method described here is the first one available to estimate the microbial metaldehyde degradation potential and activity in soils, and can also be used to detect degrading microorganisms in systems such as sand filters for water purification or to monitor degrading strains in engineered processes. Previous article in issuees_ES
dc.description.sponsorshipUniversidad de Costa Rica/[]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de York/[]/UY/Reino Unidoes_ES
dc.language.isoenges_ES
dc.sourceJournal of Microbiological Methods; Vol. 195: 2022es_ES
dc.subjectqPCRes_ES
dc.subjectDegrading genees_ES
dc.subjectMetaldehydees_ES
dc.subjectSoiles_ES
dc.subjectPESTICIDESes_ES
dc.subjectBACTERIAes_ES
dc.titleProbe-based qPCR assay enables the rapid and specific detection of bacterial degrading genes for the pesticide metaldehyde in soiles_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/j.mimet.2022.106447
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro en Investigación en Contaminación Ambiental (CICA)es_ES


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