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dc.creatorArias Arias, Jorge Luis
dc.creatorMora Rodríguez, Rodrigo Antonio
dc.date.accessioned2022-07-20T13:51:38Z
dc.date.available2022-07-20T13:51:38Z
dc.date.issued2021-10-01
dc.identifier.citationhttps://link.springer.com/shop/springer/titles/en-gb/?gclid=CjwKCAjwzeqVBhAoEiwAOrEmzXiJd8RnCiXKFoOsRNa3q0B_xUP41f4skY7ia9eNk779JnrjbBwxMhoCQA8QAvD_BwEes_ES
dc.identifier.isbn978-3-030-71165-8
dc.identifier.urihttps://hdl.handle.net/10669/86988
dc.description.abstractThe genus Flavivirus within the family Flaviviridae contains many arthropod-borne infectious agents of medical relevance like dengue virus (DENV), Zika virus (ZIKV), and West Nile virus (WNV), among others, that cause epi- demics of hemorrhagic fevers and encephalitis for which there are no antiviral treatments and effective vaccines available. Fluorescence imaging is a powerful and versatile research tool for the study of fl aviviral diseases. This tool can be com- plemented with biochemical and molecular methods to gain insight into the mechanisms of fl avivirus infection and immunity, in order to develop feasible prophylactic and therapeutic interventions to lower their impact on public health. Here we discuss the basics of the fl uorescence imaging techniques currently employed in fl avivirus research, including immuno fl uorescence assay (IFA), fl uorescence in situ hybridization (FISH), fl uorescence-labeled viral particles, fl uorescent labeling of cytopathic effect (CPE), subgenomic reporter replicons (SRRs)/reporter virus particles (RVPs), and cell-based molecular reporters (CBMRs). We also address the advantages of each application based on our own experience and included some of our protocols to facilitate its applications.es_ES
dc.language.isoenges_ES
dc.sourceSpringeres_ES
dc.subjectDengue virus (DENV)es_ES
dc.subjectZika virus (ZIKV)es_ES
dc.subjectYellow fever virus (YFV)es_ES
dc.subjectWest Nile virus (WNV)es_ES
dc.subjectFlaviviruses_ES
dc.subjectImmunofluorescence assay (IFA)es_ES
dc.subjectIn situ hybridization (FISH)es_ES
dc.subjectFluorescence-labeled viral particleses_ES
dc.subjectCytopathic effect (CPE)es_ES
dc.subjectSubgenomic repliconses_ES
dc.subjectReporter virus particles (RVPs)es_ES
dc.subjectCell-based molecular reporterses_ES
dc.subjectViral plaqueses_ES
dc.subjectLive-cell imaginges_ES
dc.titleFluorescence Imaging Approaches in Flavivirus Researches_ES
dc.typecapítulo de libroes_ES
dc.identifier.doihttps://doi.org/10.1007/978-3-030-71165-8_34
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES


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