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dc.creatorNavarro Hoyos, Mirtha
dc.creatorArnáez Serrano, Elizabeth
dc.creatorQuesada Mora, Silvia
dc.creatorAzofeifa Cordero, Gabriela
dc.creatorWilhelm Romero, Krissia
dc.creatorQuirós Fallas, María isabel
dc.creatorAlvarado Corella, Luis Diego
dc.creatorVargas Huertas, Luis Felipe
dc.creatorSánchez Kopper, Andrés
dc.date.accessioned2022-11-10T21:39:14Z
dc.date.available2022-11-10T21:39:14Z
dc.date.issued2021
dc.identifier.citationhttps://www.mdpi.com/1420-3049/26/23/7367es_ES
dc.identifier.issn1420-3049
dc.identifier.urihttps://hdl.handle.net/10669/87680
dc.description.abstractThere is increasing interest in research into fruits as sources of secondary metabolites because of their potential bioactivities. In this study, the phenolic profiles of Malus domestica Anna and Jonagold cultivars from Costa Rica were determined by Ultra Performance Liquid Chromatogra phy coupled with High Resolution Mass Spectrometry (HRMS) using a quadrupole-time-of-flight analyzer (UPLC-QTOF-ESI MS), on enriched-phenolic extracts from skins and flesh, obtained through Pressurized Liquid Extraction (PLE). In total, 48 different phenolic compounds were identified in the skin and flesh extracts, comprising 17 flavan-3-ols, 12 flavonoids, 4 chalcones, 1 glycosylated isoprenoid and 14 hydroxycinnamic acids and derivatives. Among extracts, the flesh of Jonagold exhibits a larger number of polyphenols and is especially rich in procyanidin trimers, tetramers and pentamers. Evaluating total phenolic content (TPC) and antioxidant activities using ORAC and DPPH procedures yields higher values for this extract (608.8 mg GAE/g extract; 14.80 mmol TE/g extract and IC50 = 3.96 µg/mL, respectively). In addition, cytotoxicity evaluated against SW620 colon cancer cell lines and AGS gastric cancer cell lines also delivered better effects for Jonagold flesh (IC50 = 62.4 and 60.0 µg/mL, respectively). In addition, a significant negative correlation (p < 0.05) was found between TPC and cytotoxicity values against SW620 and AGS adenocarcinoma (r = −0.908, and −0.902, respectively). Furthermore, a significant negative correlation (p < 0.05) was also found between the number of procyanidins and both antioxidant activities and cytotoxicity towards SW620 (r = −0.978) and AGS (r = −0.894) cell lines. These results align with Jonagold flesh exhibiting the highest abundance in procyanidin oligomers and yielding better cytotoxic and antioxidant results. In sum, our findings suggest the need for further studies on these Costa Rican apple extracts—and particularly on the extracts from Jonagold flesh—to increase the knowledge on their potential benefits for health.es_ES
dc.description.sponsorshipUniversidad de Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourceMolecules, 26(23).es_ES
dc.subjectMalus domesticaes_ES
dc.subjectAPPLEes_ES
dc.subjectUPLCes_ES
dc.subjectESI-MSes_ES
dc.subjectmass spectrometryes_ES
dc.subjectpolyphenolses_ES
dc.subjectflavonoidses_ES
dc.subjectprocyanidinses_ES
dc.subjectnutraceutices_ES
dc.subjectantioxidantes_ES
dc.subjectantitumorales_ES
dc.subjectFRUITes_ES
dc.subjectCOSTA RICAes_ES
dc.subjectCHEMICAL ANALYSISes_ES
dc.subjectMEDICINAL PLANTSes_ES
dc.titleHRMS characterization, antioxidant and cytotoxic activities of polyphenols in Malus domestica cultivars from Costa Ricaes_ES
dc.typeartículo originales_ES
dc.identifier.doi10.3390/molecules26237367
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químicaes_ES
dc.identifier.codproyecto115-B2-657
dc.identifier.codproyectoED-3239
dc.identifier.codproyecto115-B0-047
dc.identifier.codproyectoED-2033


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