Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
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Date
Authors
Bolaños Villegas, Pablo Alberto
Yang, Xiaohui
Makaroff, Christopher A.
Jauh, Guang-Yuh
Journal Title
Journal ISSN
Volume Title
Publisher
Bio-protocol 4(7), e1102, 2014
Abstract
This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes.
Description
artículo -- Universidad de Costa Rica. Escuela de Agronomía, 2014
Keywords
Meiosis, Arabidopsis thaliana, Cromosomas, Hibridización in situ fluorescente
Citation
http://www.bio-protocol.org/e1102