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Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization

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Authors

Bolaños Villegas, Pablo Alberto
Yang, Xiaohui
Makaroff, Christopher A.
Jauh, Guang-Yuh

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Bio-protocol 4(7), e1102, 2014

Abstract

This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes.

Description

artículo -- Universidad de Costa Rica. Escuela de Agronomía, 2014

Keywords

Meiosis, Arabidopsis thaliana, Cromosomas, Hibridización in situ fluorescente

Citation

http://www.bio-protocol.org/e1102

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