Microbiología
Envíos recientes
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Implementación de un laboratorio de análisis proteómicos en el Instituto Clodomiro Picado (UCR): primeras experiencias en Costa Rica
(2012)Reseña sobre avances tecnológicos en la implementación de un laboratorio de análisis proteómico en Costa Rica. Se describe la espectrometría de masas, la ionización de muestra, la instrumentación básica, la espectrometría ... -
Mipartoxin-I, a novel three-finger toxin, is the major neurotoxic component in the venom of the redtail coral snake Micrurus mipartitus (Elapidae)
(2012)The major venom component of Micrurus mipartitus, a coral snake distributed from Nicaragua to northern South America, was characterized biochemically and functionally. This protein, named mipartoxin-I, is a novel member ... -
Identification of linear B-cell epitopes on myotoxin-II, a Lys49 phospholipase A2 homologue from Bothrops asper snake venom
(2012)Knowledge on toxin immunogenicity at the molecular level can provide valuable information for the improvement of antivenoms, as well as for understanding toxin structure– function relationships. The aims of this study ... -
Snake venom Lys49 myotoxins: from phospholipases A2 to non-enzymatic membrane disruptors
(2012-09)Snake venoms often contain toxins that cause a rapid necrosis of skeletal muscle fibers, referred to as myotoxins. The most common among them are phospholipases A2 (PLA2s), enzymes that have two independent evolutionary ... -
An acidic phospholipase A2 with antibacterial activity from Porthidium nasutum snake venom
(2012-04)Snake venoms are complex mixtures of proteins among which both basic and acidic phospholipases A2 (PLA2s) can be found. Basic PLA2s are usually responsible for major toxic effects induced by snake venoms, while acidic ... -
Resurrexit, sicut dixit, alleluia. Snake venomics from a 26-years old polyacrylamide focusing gel
(2012)A 26-year-old dried polyacrylamide gel, cast in presence of an immobilized pH gradient and containing focused proteins from the venoms of a northern black-tailed rattlesnake (Crotalus molossus molossus), and of a western ... -
Mortality by Hymenoptera stings in Costa Rica, 1985-2006
(2009)Objective. To analyze mortality due to Hymenoptera stings in Costa Rica during 1985–2006. Methods. Records of deaths due to Hymenoptera stings in 1985–2006 were retrieved from Instituto Nacional de Estadística y Censos ... -
Biochemistry and toxicology of toxins purified from the venom of the snake Bothrops asper
(2009-12-01)The isolation and study of individual snake venom components paves the way for a deeper understanding of the pathophysiology of envenomings – thus potentially contributing to improved therapeutic modalities in the clinical ... -
Neutralization of Bothrops asper venom by antibodies, natural products, and synthetic drugs: contributions to understanding snakebite envenomings and their treatment
(2009)Interest in studies on the neutralization of snake venoms and toxins by diverse types of inhibitors is two-fold. From an applied perspective, results enclose the potential to be translated into useful therapeutic products ... -
Acute physiopathological effects of honeybee (Apis mellifera) envenoming by subcutaneous route in a mouse model
(2010)Bee stings are a health concern in the Americas, where fatal envenomings due to massive attacks by Africanized honeybees have been documented in the last decades. Most studies on the toxic effects of honeybee venom in ... -
Synthetic peptides derived from the C-terminal region of Lys49 phospholipase A2 homologues from Viperidae snake venoms: biomimetic activities and potential applications
(2010)Lys49-phospholipase A2 homologues constitute a large family of toxins present in the venoms of viperid snake species, which despite lacking catalytic activity, cause significant skeletal muscle necrosis. The main structural ... -
Venom, venomics, antivenomics
(2009)Venoms comprise mixtures of peptides and proteins tailored by Natural Selection to act on vital systems of the prey or victim. Here we review our proteomic protocols for uncoiling the composition, immunological profile, ... -
Cytotoxicity of Lachesis muta muta snake (bushmaster) venom and its purified basic phospholipase A2 (LmTX-I) in cultured cells
(2007)Human envenoming by Lachesis muta muta venom, although infrequent, is rather severe, being characterized by pronounced local tissue damage and systemic dysfunctions. Studies on the pharmacological actions of L. m. muta ... -
Antitumor effects of cationic synthetic peptides derived from Lys49 phospholipase A2 homologues of snake venoms
(2007)The effects of two cationic synthetic peptides, derived from the C-terminal region of Lys49 phospholipase A2 homologues from snake venoms, upon various murine tumor cell lines (B16 melanoma, EMT6 mammary carcinoma, S-180 ... -
Isolation, characterization and molecular cloning of AnMIP, a new α-type phospholipase A2 myotoxin inhibitor from the plasma of the snake Atropoides nummifer (Viperidae: Crotalinae)
(2007)A new phospholipase A2 (PLA2)-inhibitory protein was isolated from the plasma of Atropoides nummifer, a crotaline snake from Central America. This inhibitor was named AnMIP, given its ability to neutralize the activity ... -
Biological and structural characterization of crotoxin and new isoform of crotoxin B PLA2 (F6a) from Crotalus durissus collilineatus snake venom
(2006)A new crotoxin B isoform PLA2 (F6a), from Crotalus durissus collilineatus was purified from by one step reverse phase HPLC chromatography using l-Bondapack C-18 column analytic. The new crotoxin B isoform PLA2 (F6a), ... -
Catalytically inactive phospholipase A2 homologue binds to vascular endothelial growth factor receptor-2 via C-terminal loop region
(2008)VEGF (vascular endothelial growth factor) regulates neovascularization through binding to its receptorKDR(kinase insert domaincontaining receptor; VEGF receptor-2). We recently identified a catalytically inactive PLA2 ... -
Computational Biology in Costa Rica: the role of a small country in the global context of bioinformatics
(2008)Introduction: The successful development of high throughput methods for DNA sequencing, transcriptomics, proteomics, and other –omics, has contributed to the emergence of novel possibilities for the examination of complex ... -
Ability of fucoidan to prevent muscle necrosis induced by snake venom myotoxins: comparison of high- and low-molecular weight fractions
(2008)Fucoidan, a natural polysaccharide extracted from brown seaweed, inhibits the myotoxic phospholipases A2 present in the venoms of crotalid snakes. This study evaluated the influence of molecular weight on the ability of ... -
Human heterophilic antibodies against equine immunoglobulins: assessment of their role in the early adverse reactions to antivenom administration
(2008)The presence of human heterophilic antibodies against horse immunoglobulins (HHA-HI) was determined by ELISA in sera from healthy volunteers and from patients who received equine antivenom for therapy of snake bite ...
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