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dc.creatorGutiérrez, José Maríaes_ES
dc.creatorLomonte, Brunoes_ES
dc.creatorChaves Mora, Fernandoes_ES
dc.creatorMoreno Robles, Edgardoes_ES
dc.creatorCerdas Fallas, Luises_ES
dc.date.accessioned2016-11-03T22:35:28Zes_ES
dc.date.available2016-11-03T22:35:28Zes_ES
dc.date.issued1986es_ES
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/0742841386901830es_ES
dc.identifier.issn0306-4492es_ES
dc.identifier.urihttp://hdl.handle.net/10669/29143es_ES
dc.description.abstractA toxic phospholipase A was isolated from the venom of Bothrops asper. It induced skeletal muscle damage, anticoagulant effects and edema in the foot pad. The toxin had an intravenous LD50 of 95 micrograms/16-18 g mouse body wt and an intraventricular LD50 of 0.42 micrograms/16-18 g mouse body wt. Upon intramuscular and intravenous injections, the toxin induced a prominent increase in serum creatine kinase (CK) levels; only the CK-MM isozyme increased markedly. The toxin induced CK and creatine release from skeletal muscle incubated in vitro. The rate of efflux of creatine was higher than that of CK, although both markers were partially released as early as 15 min after incubation. The toxin also induced elevation of serum levels of lactic dehydrogenase isozymes. However, histological examination of skeletal muscle, kidneys, heart and lungs revealed cell damage only in skeletal muscle. The toxin was not cytotoxic to erythrocytes, lymphocytes or macrophages. In addition, it did not induce a mitogenic response on lymphocytes. In the absence of albumin in the medium, there was no significant difference between myotoxic activities in Ca2+-free and Ca2+-containing bathing solutions. However, when albumin was added, there was a significantly higher myotoxic effect in the presence of Ca2+. Thus, although phospholipolytic activity of the toxin plays a role in muscle damage when albumin is present, the toxin induces muscle damage even when phospholipase A activity is inhibited.es_ES
dc.language.isoen_USes_ES
dc.sourceComparative Biochemistry and Physiology Part C: Comparative Pharmacology, Volumen 84, Número 1, 1986es_ES
dc.subjectAnimalses_ES
dc.subjectCell Survivales_ES
dc.subjectCreatine Kinasees_ES
dc.subjectCrotalid Venomses_ES
dc.subjectEdemaes_ES
dc.subjectIn Vitro Techniqueses_ES
dc.subjectL-Lactate Dehydrogenasees_ES
dc.subjectKineticses_ES
dc.subjectIsoenzymeses_ES
dc.subjectSpecies Specificityes_ES
dc.subjectPhospholipases Aes_ES
dc.subjectMitogenses_ES
dc.titlePharmacological activities of a toxic phospholipase A isolated from the venom of the snake Bothrops asperes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/0742-8413(86)90183-0es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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