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dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorRojas Céspedes, Gustavo
dc.creatorCalderón Fuentes, Leonel
dc.date.accessioned2016-11-09T21:56:33Z
dc.date.available2016-11-09T21:56:33Z
dc.date.issued1991
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/004101019190061Ues_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29168
dc.description.abstractAn enzyme-immunoassay (EIA) for the quantitation of antibodies against myotoxins present in the venoms of Bothrops asper (Costa Rica), B. atrox (Colombia) and B. moojeni (Brazil), was developed. This EIA was utilized for the evaluation of four antivenoms produced in Mexico (Laboratorios Myn; MYN), Costa Rica (Instituto Clodomiro Picado; ICP), Colombia (Instituto Nacional de Salud; INS) and Brazil (Instituto Butantan; IB). Antivenoms ICP, IB and INS showed a higher titer of antibodies against the three myotoxins tested, with only slight differences between them, depending on the antigen utilized. In contrast, MYN antivenom had very low levels of antibodies to the three myotoxins. Seventeen batches of ICP antivenom were analyzed by EIA, using B. asper myotoxin II as antigen. Although all batches had high anti-myotoxin titers, these varied significantly. Batches produced after 1988 had, in general, higher titers than older (1986-1987) ones. Antivenom stored for one year at 37 degrees C had a slight, but significant (P less than 0.002) decrease of anti-myotoxin activity, compared to antivenom stored at 4 degrees C or 23 degrees C. No correlation was found between anti-myotoxin concentration and lethality-neutralizing ability of the ICP antivenom.es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon Volumen 29, Número 6, 1991es_ES
dc.subjectAnimalses_ES
dc.subjectAntibodieses_ES
dc.subjectAntiveninses_ES
dc.subjectChromatography, Ion Exchangees_ES
dc.subjectCrotalid Venomses_ES
dc.subjectDrug Storagees_ES
dc.subjectHorseses_ES
dc.subjectImmunoenzyme Techniqueses_ES
dc.subjectMuscular Diseaseses_ES
dc.subjectTemperaturees_ES
dc.titleQuantitation by enzyme-immunoassay of antibodies against Bothrops myotoxins in four commercially-available antivenomses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/0041-0101(91)90061-U
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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