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dc.creatorFernandes, Irene
dc.creatorLima, E.X
dc.creatorTakehara, Harumi Ando
dc.creatorMoura Da Silva, Ana M.
dc.creatorTancioni, Isabelle
dc.creatorGutiérrez, José María
dc.date.accessioned2017-01-24T19:40:36Z
dc.date.available2017-01-24T19:40:36Z
dc.date.issued2000-05
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010199001774es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29462
dc.description.abstractHorse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Rica. Toxicon 000–000. This work compared the specificity, ELISA titers and IgG subclass content of the polyvalent antivenom (anti-Bothrops asper, Crotalus durissus durissus and Lachesis muta stenophrys) of Instituto Clodomiro Picado (Costa Rica) and the bothropic antivenom (anti-Bothrops jararaca, B. jararacussu, B. moojeni, B. neuwiedi and B. alternatus) of Instituto Butantan (Brazil). The role of IgG(T) and IgGa subclasses in neutralization of some venom toxic activities and the cross neutralization of the antivenoms against B. jararaca and B. asper venoms were also evaluated. Both antivenoms were able to recognize B. asper and B. jararaca venoms by immunoblotting and presented similar antibody titers when assayed by ELISA. IgG(T) was highest, followed by IgGa, IgGb and IgGc. IgGa and IgG(T) isotypes isolated from both antivenoms by affinity chromatography were tested for neutralization of lethal, hemorrhagic, coagulant and phospholipase A2 activities of the homologous venoms. In both antivenoms, IgG(T) was the major isotype responsible for neutralization of all the tested activities, followed by IgGa. These results suggest that Instituto Butantan and Instituto Clodomiro Picado antivenoms have the same IgG profile and their neutralizing ability is due mostly to the IgG(T) isotype. Also, they neutralize lethality in mice induced by homologous and heterologous venoms, the bothropic antivenom of Instituto Butantan being more effective.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-89-057]/UCR/Costa Ricaes_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo//FAPESP/Brasiles_ES
dc.description.sponsorshipEuropean Community/[CI1-CT94-0043)//Internacionales_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico//CNPq/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 38, Número 5, 2000es_ES
dc.subjectIgG isotypeses_ES
dc.subjectCross-neutralizationes_ES
dc.subjectSnake antivenomes_ES
dc.subjectHorsees_ES
dc.subjectSnake venomes_ES
dc.titleHorse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Ricaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/S0041-0101(99)00177-4
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-89-057


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