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dc.creatorGutiérrez, José María
dc.creatorTsai, Wan-Chih
dc.creatorPla Ferrer, Davinia
dc.creatorSolano Trejos, María Gabriela
dc.creatorLomonte, Bruno
dc.creatorSanz, Libia
dc.creatorAngulo Ugalde, Yamileth
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2017-06-09T14:32:39Z
dc.date.available2017-06-09T14:32:39Z
dc.date.issued2013-03-15
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010113000093es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/30078
dc.description.abstractA polyspecific antivenom is used in Central America for the treatment of envenomings by viperid snakes. This antivenom is generated in horses hyperimmunized with a mixture of venoms from Bothrops asper, Crotalus simus and Lachesis stenophrys. The present study analyzed the ability of this antivenom to neutralize the venoms of three Central American viperid species of the ‘Porthidium group’, i.e. Porthidium nasutum, Porthidium ophryomegas and Cerrophidion sasai, formerly classified as Cerrophidion godmani. In addition, the immunorecognition of the components of these venoms was assessed by immunoaffinity antivenomics. The antivenom proved effective in neutralizing the lethal, hemorrhagic, myotoxic, phospholipase A2 (PLA2) and proteinase activities of the three venoms, albeit exhibiting quantitative differences in the values of the Median Effective Doses (ED50). Excepting for certain low molecular mass bands corresponding to disintegrins, and some PLA2s and PI-metalloproteinases, Western blotting and immunoaffinity chromatography revealed immunorecognition of most Porthidium and Cerrophidion venom proteins. In agreement with in vivo neutralization assays, immobilized antivenom IgGs showed higher immunocapturing activity of toxins from both Porthidium taxa than from C. sasai. Overall our results demonstrate a significant paraspecific protection of the Costa Rican polyspecific antivenom against the three venoms sampled. They also stress the need to search for novel ways to enhance the immune response of horses against several weakly immunogenic venom componentses_ES
dc.description.sponsorshipConsejo Nacional De Rectores//CONARE/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia y Tecnología/108/CONICIT/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B0-519]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-652]/UCR/Costa Ricaes_ES
dc.description.sponsorshipPrograma Iberoamericano de Ciencia y Tecnología para el Desarrollo/[BIOTOX 212RT0467]/CYTED/Españaes_ES
dc.description.sponsorshipMinisterio de Economía y Competitividad/[BFU2010-17373]//Españaes_ES
dc.description.sponsorshipGeneralitat Valenciana/[PROMETEO/2010/005]//Españaes_ES
dc.description.sponsorshipFundación Costa Rica - Estados Unidos para la Cooperación/[2009CR0021]/CRUSA-CSIC/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 64. 2013es_ES
dc.subjectSnake venomes_ES
dc.subjectPorthidiumes_ES
dc.subjectCerrophidiones_ES
dc.subjectAntivenomes_ES
dc.subjectAntivenomicses_ES
dc.subjectNeutralizationes_ES
dc.titlePreclinical assessment of a polyspecific antivenom against the venoms of Cerrophidion sasai, Porthidium nasutum and Porthidium ophryomegas: Insights from combined antivenomics and neutralization assayses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2012.12.024
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid23313380


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