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dc.creatorRighetti, Pier Giorgio
dc.creatorLomonte, Bruno
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2018-04-18T20:06:07Z
dc.date.available2018-04-18T20:06:07Z
dc.date.issued2012
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S1874391911004751es_ES
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10669/74491
dc.description.abstractA 26-year-old dried polyacrylamide gel, cast in presence of an immobilized pH gradient and containing focused proteins from the venoms of a northern black-tailed rattlesnake (Crotalus molossus molossus), and of a western diamondback rattlesnake (Crotalus atrox) has been screened in order to see the feasibility of extracting the proteins, analyzing thembymass spectrometry (MS) and assessing their integrity.Nine gel bandswere excised along the pH 3–10 gradient and the gel segments reswollen inwarmacetonitrile. Upon digestion andMSanalysis, all the bands could be identified and attributed to the respective venoms of the two rattlesnake species. Although a few peptides exhibited modified amino acids, the proteins were found to be well preserved even upon such a long storage at room temperature. The present data suggest the feasibility of identifying proteins from very old samples trapped in polyacrylamide gels, and analyzed in a pre-mass spectrometry era, thus of uncertain identity.es_ES
dc.description.sponsorshipMinistry of Education, University and Research/[RIN2008-20087ATS57]/MIUR/Italiaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[BFU2010-173]/MICINN/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteomics, vol. 75(3), p.1074-1078 (2012)es_ES
dc.subjectSnake venomicses_ES
dc.subjectDried polyacrylamide gelses_ES
dc.subjectProtein recoveryes_ES
dc.subjectMass spectrometryes_ES
dc.subjectImmobilized pH gradientses_ES
dc.subjectVenómicaes_ES
dc.subjectVenomicses_ES
dc.subjectProteómicaes_ES
dc.subjectProteomicses_ES
dc.titleResurrexit, sicut dixit, alleluia. Snake venomics from a 26-years old polyacrylamide focusing geles_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.jprot.2011.09.021
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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