Mechanical transmission of maize rayado fino marafivirus (MRFV) to maize and barley by means of the vascular puncture technique
artículo original
Ver/
Fecha
2000-04Autor
Madriz Ordeñana, Kenneth
Rojas Montero, Roy
Lundsgaard, Torben
Ramírez Fonseca, Pilar
Thordal Christensen, Hans
Collinge, David B.
Metadatos
Mostrar el registro completo del ítemResumen
Maize rayado fino marafivirus (MRFV) was mechanically transmitted to kernels of three Costa Rican maize cultivars by the vascular puncture technique. A transmission rate of up to 43.2% was obtained for cultivar HC‐57. The rate of MRFV transmission to maize was possibly affected by the morphology of the kernel, which may influence physical access to the vascular tissue after water imbibition. Transmission to maize was confirmed by western blot and immunosorbent electron microscopy. By slight modifications of the procedure, MRFV was also transmitted to barley. This is the first report of MRFV infecting a species outside the supertribe Andropogonodae. Virus infection and replication in barley were confirmed by RT‐PCR detection of MRFV RNA and by detection of the virus by ELISA
External link to the item
10.1046/j.1365-3059.2000.00447.x
Copyright 2000, British Society for Plant Pathology. Datos incluidos por Lisela Moreira Carmona, con autorizacion de Dr. Kenneth Madriz Ordeñana (junio 2019)
Colecciones
- Agronomía [1485]
El ítem tiene asociados los siguientes ficheros de licencia: