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Técnicas de Laboratorio en Inmunología Clínica
(Universidad de Costa Rica. Sede Rodrigo Facio, 2009, 2009)
El presente manual pretende proporcionar al estudiante de la carrera de Microbiología y Química Clínica la información esencial para comprender, ejecutar e interpretar un conjunto de técnicas inmunológicas de aplicación ...
Manual de Métodos Inmunológicos
(Universidad de Costa Rica. Sede Rodrigo Facio, 2007, 2020)
El presente Manual tiene como objetivo proporcionar al lector la información esencial para comprender, ejecutar e interpretar los principales tipos de técnicas inmunológicas básicas en el laboratorio. Las aplicaciones ...
Neutrophils as Trojan Horse Vehicles for Brucella abortus Macrophage Infection
(2019-05-07)
Brucella abortus is a stealthy intracellular bacterial pathogen of animals and humans. This bacterium promotes the premature cell death of neutrophils (PMN) and resists the killing action of these leukocytes. B. abortus-infected ...
Intracellular passage triggers a molecular response in that increases its infectiousness
(2021)
Brucella abortus is a facultative extracellular-intracellular pathogen that encounters a diversity of environments within the host cell. We report that bacteria extracted from infected cells at late stages (48 h post-infection) ...
Two-dimensional gel electrophoresis (2D-GE) image analysis based on CellProfiler: Pseudomonas aeruginosa AG1 as model
(2020)
Two-dimensional gel electrophoresis (2D-GE) is an indispensable technique for the study of proteomes of biological systems,
providing an assessment of changes in protein abundance under various experimental conditions. ...
A first perturbome of Pseudomonas aeruginosa: Identification of core genes related to multiple perturbations by a machine learning approach
(2021)
Tolerance to stress conditions is vital for organismal survival, including bacteria under specific environmental
conditions, antibiotics, and other perturbations. Some studies have described common modulation and shared
genes ...
Predicting Cancer Chemosensitivity Based on Intensity/Distribution Profiles of Cells Loaded with a Fluorescent Sphingolipid Analogue
(2018)
Cancer is a group of heterogeneous and complex
diseases, with limited therapeutic options due to the recurrent
emergence of drug resistance. Sphingolipids are bioactive
molecules that participate in signaling of cell ...
A hybrid mathematical modeling approach of the metabolic fate of a fluorescent sphingolipid analogue to predict cancer chemosensitivity
(2018-06)
Sphingolipid (SL) metabolism is a complex biological system that produces and transforms ceramides and other molecules able to modulate other cellular processes, including survival or death pathways key to cell fate ...
Set-theory based benchmarking of three different variant callers for targeted sequencing
(2021)
Background: Next generation sequencing (NGS) technologies have improved the
study of hereditary diseases. Since the evaluation of bioinformatics pipelines is not
straightforward, NGS demands efective strategies to analyze ...
Molecular Determinants of Antibiotic Resistance in the Costa Rican Pseudomonas aeruginosa AG1 by a Multi-omics Approach: A Review of 10 Years of Study
(2021)
Pseudomonas aeruginosa AG1 (PaeAG1) is a Costa Rican strain that was isolated in 2010 in a major Hospital. This strain
has resistance to multiple antibiotics such as β-lactams (including carbapenems), aminoglycosides, and ...