Listar Microbiología por autor "87486222-cf63-4400-bfbb-f85b2ffbefcf"
Mostrando ítems 1-12 de 12
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A cellular deficiency of gangliosides causes hypersensitivity to Clostridium perfringens phospholipase C
Flores Díaz, Marietta; Alape Girón, Alberto; Clark, Graeme; Catimel, Bruno; Hirabayashi, Yoshio; Nice, Ed; Gutiérrez, José María; Titball, Richard; Thelestam, Mónica (2005-07-22)Clostridium perfringens phospholipase C (Cp-PLC), also called alpha-toxin, is the major virulence factor in the pathogenesis of gas gangrene. Previously, a cellular UDP-Glc deficiency was related with a hypersensitivity ... -
Development of immunoassays for determination of circulating venom antigens during envenomations by coral snakes (Micrurus species)
Amuy, Ernesto; Lomonte, Bruno; Thelestam, Mónica; Gutiérrez, José María; Alape Girón, Alberto (1997-11)A reverse agglutination assay and two capture enzyme-linked immunoassays (ELISAs) for the quantitative determination of Micrurus nigrocinctus nigrocinctus venom antigens in fluids were developed using affinity-purified ... -
Effect of Bothrops asper (Fer-De-Lance) snake venom on erythrocyte membrane. A comparative study
Quirós, Shirley; Gené, José Antonio; Gutiérrez, José María; Thelestam, Mónica (1992-02)1. The effect of Bothrops asper venom on erythrocytes of different species, sheep erythrocyte ghost vesicles and liposomes made of phospholipids from sheep erythrocytes was studied. 2. B. asper venom had a direct hemolytic ... -
Effects of Bothrops asper (terciopelo) myotoxin III, a basic phospholipase A2, on liposomes and mouse gastrocnemius muscle
Bultrón, Euris; Gutiérrez, José María; Thelestam, Mónica (1993-02)The action of Bothrops asper myotoxin III, a basic phospholipase A2 which induces acute muscle damage, was studied in mouse gastrocnemius muscle in vivo and in vitro and in multilamellar liposomes. Myonecrosis occurred ... -
Effects on cultured mammalian cells of myotoxin III, a phospholipase A2 isolated from Bothrops asper (terciopelo) venom
Bultrón, Euris; Thelestam, Mónica; Gutiérrez, José María (1993-11)Myotoxin III (MT-III), a myotoxic phospholipase A2 from Bothrops asper, was studied with respect to interactions with cultured mammalian cells and red blood cells. Tests of the cytopathogenic effect of MT-III on different ... -
Elapid venom toxins: multiple recruitments of ancient scaffolds
Alape Girón, Alberto; Persson, Bengt; Cederlund, Ella; Flores Díaz, Marietta; Gutiérrez, José María; Thelestam, Mónica; Bergman, Tomas; Jörnvall, Hans (1999-01)Nigroxins A and B, two myotoxic phospholipases A2 (PLA2s) from the venom of the American elapid Micrurus nigrocinctus, belong to a new PLA2 subclass. Their primary structures were established and compared with those of ... -
Electrophoretic and immunochemical studies of Micrurus venoms
Alape Girón, Alberto; Lomonte, Bruno; Gustafsson, Bjorn; Da Silva, Nelson Jorge; Thelestam, Mónica (1994)The electrophoretic mobilities of venom components from 15 Micrurus species were studied by polyacrylamide gel electrophoresis. The venoms showed species-specific protein patterns under native (PAGE) or denaturing (urea-PAGE) ... -
Identification of residues critical for toxicity in Clostridium perfringens phospholipase C, the key toxin in gas gangrene
Alape Girón, Alberto; Flores Díaz, Marietta; Guillouard, Isabelle; Naylor, Claire E.; Titball, Richard; Rucavado Romero, Alexandra; Lomonte, Bruno; Basak, Ajit K.; Gutiérrez, José María; Cole, Steward T.; Thelestam, Mónica (2000-08)Clostridium perfringens phospholipase C (PLC), also called α-toxin, is the major virulence factor in the pathogenesis of gas gangrene. The toxic activities of genetically engineered α-toxin variants harboring single ... -
Immunochemical characterization of Micrurus nigrocinctus nigrocinctus venom with monoclonal and polyclonal antibodies
Alape Girón, Alberto; Gustafsson, Bjorn; Lomonte, Bruno; Thelestam, Mónica; Gutiérrez, José María (1994)Eleven murine monocloncal antibodies (MAbs) against Micrurus nigrocinctus nigrocinctus venom were produced and partially characterized. When M. n. nigrocinctus venom proteins were separated by SDS-PAGE under non-reducing ... -
Increased Infectivity of Staphylococcus aureus in an Experimental Model of Snake Venom–Induced Tissue Damage
Saravia Otten, Patricia; Gutiérrez, José María; Arvidson, Staffan; Thelestam, Mónica; Flock, Jan-Ingmar (2007-09-01)Soft-tissue infection is commonly found in patients bitten by Latin American Bothrops snakes. Staphylococcus aureus, which is not present in the mouth of the snake, is frequently isolated from these infections. The effects ... -
Membrane independent activation of fibroblast proMMP-2 by snake venom: novel roles for venom proteinases
Saravia Otten, Patricia; Frisan, Teresa; Thelestam, Mónica; Gutiérrez, José María (2004-12-01)ProMMP-2 activation by Bothrops asper venom was investigated in mouse gastrocnemius muscle, mammalian cell culture and a cell-free system. Zymography revealed an increment of latent and activated forms of MMP-2 in muscle ... -
Venom of the crotaline snake Atropoides nummifer (jumping viper) from Guatemala and Honduras: comparative toxicological characterization, isolation of a myotoxic phospholipase A(2) homologue and neutralization by two antivenoms
Rojas Umaña, Ermila; Saravia Otten, Patricia; Angulo Ugalde, Yamileth; Arce, Viviana; Lomonte, Bruno; Chávez, Juan José; Velásquez, Rubén; Thelestam, Mónica; Gutiérrez, José María (2001-07)A comparative study was performed on the venoms of the crotaline snake Atropoides nummifer from Guatemala and Honduras. SDS-polyacrylamide gel electrophoresis, under reducing conditions, revealed a highly similar pattern ...