Listar Microbiología por título
Mostrando ítems 1035-1054 de 1169
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Structural and functional characterization of myotoxin I, a Lys49 phospholipase A(2) homologue from Bothrops moojeni (Caissaca) snake venom
(2000-01-01)Myotoxin-I (MjTX-I) was purified to homogeneity from the venom of Bothrops moojeni by ion-exchange chromatography on CM-Sepharose. Its molecular weight, estimated by SDS–PAGE, was 13,400 (reduced) or 26,000 (unreduced). ... -
Structural and functional characterization of myotoxin I, a Lys49 phospholipase A2 homologue from the venom of the snake Bothrops atrox
(2004-07)A new myotoxin was isolated from the venom of Bothrops atrox from Colombia. B. atrox myotoxin I is a homodimer, with a subunit molecular mass of 13,826, and a pI of 8.9. Its complete nucleotide sequence was obtained by ... -
Structural and functional properties of BaTX, a new Lys49 phospholipase A2 homologue isolated from the venom of the snake Bothrops alternatus
(2007-04)BaTX PLA2, a K49 phospholipase A2 homologue was purified from Bothrops alternatus venom after two chromatographic steps, molecular exclusion on Superdex 75 and reverse phase HPLC on μ-Bondapack C-18. A molecular mass of ... -
Structural basis for phospholipase A2-like toxin inhibition by the synthetic compound Varespladib (LY315920)
(2019)The World Health Organization recently listed snakebite envenoming as a Neglected Tropical Disease, proposing strategies to significantly reduce the global burden of this complex pathology by 2030. In this context, ... -
Structural basis of the myotoxic inhibition of the Bothrops pirajai PrTX-I by the synthetic varespladib
(2023)Varespladib (LY315920) is a potent inhibitor of human group IIA phospholipase A2 (PLA2) originally developed to control inflammatory cascades of diseases associated with high or dysregulated levels of endogenous PLA2. ... -
Structural characterization and phylogenetic relationships of myotoxin II from Atropoides (Bothrops) nummifer snake venom, a Lys49 phospholipase A2 homologue
(2002)In order to analyze its structure–function relationships, the complete amino acid sequence of myotoxin II from Atropoides (Bothrops) nummifer from Costa Rica was determined. This toxin is a Lys49-type phospholipase A2 ... -
Structure of a calcium-independent phospholipase-like myotoxic protein from Bothrops asper venom
(1995-05-01)Myotoxin II, a myotoxic calcium-independent phospholipase-like protein isolated from the venom of Bothrops asper, possesses no detectable phospholipase activity. The crystal structure has been determined and refined at 2.8 ... -
Structure of a Lys49-Phospholipase A2 homologue isolated from the venom of Bothrops nummifer (jumping viper)
(1999-02)Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. We have solved the structure of myotoxin-I, a Lys49-PLA2 homologue isolated from ... -
Structure of myotoxin-II, a catalytically inactive Lys49 phospholipase A2 homologue from Atropoides nummifer venom
(2006)Lys49 snake-venom phospholipase A2 (PLA2) homologues are highly myotoxic proteins which, although lacking catalytic activity, possess the ability to disrupt biological membranes, inducing significant muscle-tissue loss ... -
Study of the design and analytical properties of the lethality neutralization assay used to estimate antivenom potency against Bothrops asper snake venom
(2010-09)The lethality neutralization assay performed in mice is the standard recommended by the World Health Organization to estimate antivenom potency. The interpretation of its results without considering its analytical capacity ... -
Sucesión de larvas de muscoideos durante la degradación cadavérica en un bosque premontano húmedo tropical
(2005-06)INTRODUCCIÓN. La sucesión de larvas de muscoideos, reconocida herramienta en las investigaciones médico legales, fue estudiada como un criterio en la estimación del intervalo post mortem (IPM) en un modelo experimental con ... -
El suero antiofídico polivalente producido en Costa Rica: Estabilidad y capacidad neutralizante
(1988)En este trabajo se revisan y discuten una serie de aspectos relativos a la producción, estabilidad y capacidad neutralizante del suero antiofídico polivalente producido por el Instituto Clodomiro Picado. Este suero ... -
El suero de neonatos de Clelia clelia (Serpentes: Colubridae) neutraliza la acción hemorrágica del veneno de Bothrops asper (Serpentes: Viperidae).
(1989)The ability of serum from nine newbom specimens of Clelia clelia (Colubridae) to neutralize hernorrhagic action of Bothrops asper venom was tested. All serum samples neutralized completely the hernorrhagic effect of the ... -
Surprising SES Gradients in Mortality, Health, and Biomarkers in a Latin American Population of Adults
(Journal of Gerontology: Social Sciences 64B(1):105-117, 2009-02-04)Background. To determine socioeconomic status (SES) gradients in the different dimensions of health among elderly Costa Ricans. Hypothesis: SES disparities in adult health are minimal in Costa Rican society. Methods. ... -
Susceptibilidad de Trypanosoma cruzi a diferentes venenos de serpientes de Costa Rica
(2008)Se evaluaron los efectos tóxicos de los venenos de cinco serpientes costarricenses en cuanto a su capacidad tripanocida contra dos cepas de Trypanosoma cruzi y sus efectos en cuanto a los mecanismos de muerte celular. ... -
Synergism between basic Asp49 and Lys49 phospholipase A2 myotoxins of viperid snake venom in vitro and in vivo
(PLoS One 9(10):e109846, 2014-10-07)Two subtypes of phospholipases A2 (PLA2s) with the ability to induce myonecrosis, ‘Asp49’ and ‘Lys49’ myotoxins, often coexist in viperid snake venoms. Since the latter lack catalytic activity, two different mechanisms are ... -
Synthetic peptides derived from the C-terminal region of Lys49 phospholipase A2 homologues from Viperidae snake venoms: biomimetic activities and potential applications
(2010)Lys49-phospholipase A2 homologues constitute a large family of toxins present in the venoms of viperid snake species, which despite lacking catalytic activity, cause significant skeletal muscle necrosis. The main structural ... -
Systemic and local myotoxicity induced by snake venom group II phospholipases A2: Comparison between crotoxin, crotoxin B and a Lys49 PLA2 homologue
(2008-01)The patterns of myotoxicity induced in mice by crotoxin, crotoxin B and a Lys49 phospholipase A2 (PLA2) homologue were compared. Lys49 PLA2-induced local myotoxicity is reflected by creatine kinase (CK) loss in injected ... -
Systemic cytokine response in children bitten by snakes in Costa Rica
(2001-12)BACKGROUND: To characterize the host response to venom from snakes of the family Viperidae in Costa Rica, we investigated the release of cytokines: IL-1, IL-6, IL-8, TNF-alpha, MIP-1beta, and RANTES in pediatric patients ... -
Systemic effects induced by the venom of the snake Bothrops caribbaeus in a murine model
(2013-03)Snakebite envenoming by Bothrops caribbaeus, an endemic viperid from the Lesser Antillean island of Saint Lucia, is clinically characterized by local tissue damage and systemic thrombosis that can lead to cerebral, myocardial ...