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dc.creatorSchrübbers, Lars C.
dc.creatorMasís Mora, Mario Alberto
dc.creatorCarazo Rojas, Elizabeth
dc.creatorValverde Mena, Bernal
dc.creatorChristensen, Jan H.
dc.creatorCedergreen, Nina
dc.date.accessioned2018-04-04T16:12:49Z
dc.date.available2018-04-04T16:12:49Z
dc.date.issued2016-01-01
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0039914015301867?via%3Dihubes_ES
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10669/74413
dc.description.abstractGlyphosate is a commonly applied herbicide in coffee plantations. Because of its non-selective mode of action it can damage the crop exposed through spray drift. Therefore, it is of interest to study glyphosate fate in coffee plants. The aim of this study was to develop an analytical method for accurate and precise quantification of glyphosate and its main metabolite aminomethylphosphonic acid (AMPA) at trace levels in coffee leaves using liquid chromatography with single-quadrupole mass spectrometry detection. The method is based on a two-step solid phase extraction (SPE) with an intermediate derivatization reaction using 9-fluorenylmethylchloroformate (FMOC). An isotope dilution method was used to account for matrix effects and to enhance the confidence in analyte identification. The limit of quantification (LOQ) for glyphosate and AMPA in coffee leaves was 41 and 111 μg kg−1 dry weight, respectively. For the method optimization a design of experiments (DOE) approach was used. The sample clean-up procedure can be simplified for the analysis of less challenging matrices, for laboratories having a tandem mass spectrometry detector and for cases in which quantification limits above 0.1 mg kg−1 are acceptable, which is often the case for glyphosate. The method is robust, possesses high identification confidence, while being suitable for most commercial and academic laboratories. All leaf samples from five coffee fields analyzed (n=21) contained glyphosate, while AMPA was absent. The simplified clean-up procedure was successfully validated for coffee leaves, rice, black beans and river water.es_ES
dc.description.sponsorshipDanish Council for Independent Research-Natural Sciences/[09 066373]/FNU/Dinamarcaes_ES
dc.language.isoen_USes_ES
dc.sourceTalanta, Vol. 146, 2016es_ES
dc.subjectGlyphosate analysises_ES
dc.subjectLiquid chromatography mass spectrometryes_ES
dc.subjectSingle quadrupolees_ES
dc.subjectCoffeees_ES
dc.subjectCoffea arabicaes_ES
dc.titleAnalysis of glyphosate and aminomethylphosphonic acid in leaves from Coffea arabica using high performance liquid chromatography with quadrupole mass spectrometry detectiones_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.talanta.2015.07.059
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro en Investigación en Contaminación Ambiental (CICA)es_ES
dc.identifier.pmid26695310


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