Snake venomics of crotalus tigris: the minimalist toxin arsenal of the deadliest neartic rattlesnake venom: evolutionary clues for generating a pan-specific antivenom against crotalid type II venoms
artículo científico

Fecha
2012-02-03Autor
Calvete Chornet, Juan José
Pérez, Alicia
Lomonte, Bruno
Sánchez, Elda
Sanz, Libia
Metadatos
Mostrar el registro completo del ítemResumen
We report the proteomic and antivenomic characterization of Crotalus tigris venom. This venom exhibits the highest lethality for mice among rattlesnakes and the simplest toxin proteome reported to date. The venom proteome of C. tigris comprises 7–8 gene products from 6 toxin families: the presynaptic β-neurotoxic heterodimeric PLA2, Mojave toxin, and two serine proteinases comprise, respectively, 66% and 27% of the C. tigris toxin arsenal, whereas a VEGF-like protein, a CRISP molecule, a medium-sized disintegrin, and 1–2 PIII-SVMPs, each represents 0.1–5% of the total venom proteome. This toxin profile really explains the systemic neuro- and myotoxic effects observed in envenomated animals. In addition, we found that venom lethality of C. tigris and other
North American rattlesnake type II venoms correlates with the concentration of Mojave toxin A subunit, supporting the view that the neurotoxic venom phenotype of crotalid type II venoms may be described as a single-allele adaptation. Our data suggest that the evolutionary trend towards neurotoxicity, which has been also reported for the South American rattlesnakes, may have resulted by paedomorphism. The ability of an experimental antivenom to effectively immunodeplete proteins from the type II venoms of C. tigris, C. horridus, C. oreganus helleri, C. scutulatus scutulatus, and S. catenatus catenatus, indicated the feasibility of generating a pan-American anti-Crotalus type II antivenom, suggested by the identification of shared evolutionary trends among South American and North American Crotalus.
External link to the item
doi:10.1021/pr201021d
artículo (arbitrado)-- Universidad de Costa Rica, Instituto de Investigaciones Clodomiro Picado. 2012.
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Proteome Research, copyright © American Chemical Society after peer review and technical editing by the publisher.
To access the final edited and published work seehttp://pubs.acs.org/doi/abs/10.1021/pr201021d
Colecciones
- Microbiología [1056]
El ítem tiene asociados los siguientes ficheros de licencia:
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Impact of Regional Variation in Bothrops asper Snake Venom on the Design of Antivenoms: Integrating Antivenomics and Neutralization Approaches
Gutiérrez, José María; Sanz, Libia; Flores Díaz, Marietta; Figueroa, Lucía; Madrigal Villalobos, Marvin; Herrera Vega, María; Villalta Arrieta, Mauren; León Montero, Guillermo; Estrada Umaña, Ricardo; Borges, Adolfo; Alape Girón, Alberto; Calvete Chornet, Juan José (2010-10-21)Intraspecific snake venom variations have implications in the preparation of venom pools for the generation of antivenoms. The impact of such variation in the cross-reactivity of antivenoms against Bothrops asper venom was ... -
Integrated “omics” profiling indicates that miRNAs are modulators of the ontogenetic venom composition shift in the Central American rattlesnake, Crotalus simus simus
Durban, Jordi; Pérez, Alicia; Sanz, Libia; Gómez Argüello, Aarón; Bonilla Murillo, Fabián; Rodríguez Arguedas, Santos; Chacón Gutiérrez, Danilo; Sasa Marín, Mahmood; Angulo Ugalde, Yamileth; Gutiérrez, José María; Calvete Chornet, Juan José (2013)Background Understanding the processes that drive the evolution of snake venom is a topic of great research interest in molecular and evolutionary toxinology. Recent studies suggest that ontogenetic changes in venom ... -
Venom variation in Bothrops asper lineages from north-western South America.
Mora Obando, Diana; Salazar Valenzuela, David; Pla Ferrer, Davinia; Lomonte, Bruno; Guerrero Vargas, Jimmy; Ayerbe, Santiago; Gibbs, H. Lisle; Calvete Chornet, Juan José (2020)Bothrops asper is a venomous pitviper that is widely distributed and of clinical importance in Mesoamerica and northern South America, where it is responsible for 50–80% of all envenomations by Viperidae species. Previous ...