Universidad de Costa Rica
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo Depositar
  • Políticas
  • Contacto
    • español
    • English
  • español 
    • español
    • English
  • Login
Ver ítem 
  •   Repositorio Kérwá
  • Investigación
  • Salud
  • Microbiología
  • Ver ítem
  •   Repositorio Kérwá
  • Investigación
  • Salud
  • Microbiología
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

Systemic effects induced by the venom of the snake Bothrops caribbaeus in a murine model

artículo científico
Ver/
Versión final (1.669Mb)
Fecha
2013-03
Autor
Herrera Arias, Cristina
Rucavado Romero, Alexandra
Warrell, David A.
Gutiérrez, José María
Metadatos
Mostrar el registro completo del ítem
Resumen
Snakebite envenoming by Bothrops caribbaeus, an endemic viperid from the Lesser Antillean island of Saint Lucia, is clinically characterized by local tissue damage and systemic thrombosis that can lead to cerebral, myocardial or pulmonary infarctions and venous thromboses. Systemic effects (lethality, pulmonary hemorrhage, thrombocytopenia and coagulopathy) induced by intravenous (i.v.) administration of B. caribbaeus venom were studied in mice. The role of snake venom metalloproteinases (SVMPs) in these systemic alterations was assessed by inhibition with the chelating agent calcium disodium ethylenediaminetetraacetic acid (CaNa2EDTA). A snake C-type lectin-like (snaclec) and a type P-III hemorrhagic SVMP were isolated and characterized from this venom, and the effect of venom and the isolated snaclec on human platelet aggregation was studied in vitro. Results indicate that SVMPs play an important role in the overall toxicity of B. caribbaeus venom, being responsible for systemic hemorrhage and lethality, but not thrombocytopenia, whereas the isolated snaclec is involved in the thrombocytopenic effect. Both venom and snaclec induce platelet aggregation/agglutination. Moreover, the snaclec binds directly to glycoprotein Ib (GPIb) and induces agglutination in washed fixed platelets. On the other hand, B. caribbaeus venom hydrolyzed fibrinogen in vitro and induced a partial drop of fibrinogen levels with an increase in fibrin/fibrinogen degradation products (FDP) levels in vivo. The negative result for D-dimer (DD) in plasma is consistent with the lack of microscopic evidence of pulmonary thrombosis and endothelial cell damage. Likewise, no increments in plasma sE-selectin levels were detected. The absence of thrombosis in this murine model suggests that this effect may be species-specific.
URI
https://hdl.handle.net/10669/30059
External link to the item
10.1016/j.toxicon.2012.10.023
http://www.sciencedirect.com/science/article/pii/S0041010112007970
Colecciones
  • Microbiología [1031]



  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
  • Repositorio Documental Rafael Obregón Loría (CIHAC)
  • Biblioteca Digital Carlos Melendez (CIHAC)
  • Repositorio de Fotografías
  • Colección de videos de UPA-VAS
  • Sitios recomendados

  • Buscador regional de LA Referencia
  • Buscador del Open ROAR
  • Scientific Electronic Library Online (SciELO)
  • Directory of Open Access Journals (DOAJ)
  • Redalyc
  • Redes sociales

  • facebook.com/repositoriokerwa
  • @Ciencia_UCR
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo depositar
  • Políticas
Contacto | Sugerencias
Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.
 

 

Listar

Todo KérwáComunidades & ColeccionesTítulosAutoresMateriasPor procedenciaPor tipoEsta colecciónTítulosAutoresMateriasPor procedenciaPor tipo

Mi cuenta

AccederRegistro

Estadísticas

Ver Estadísticas de uso

  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
  • Repositorio Documental Rafael Obregón Loría (CIHAC)
  • Biblioteca Digital Carlos Melendez (CIHAC)
  • Repositorio de Fotografías
  • Colección de videos de UPA-VAS
  • Sitios recomendados

  • Buscador regional de LA Referencia
  • Buscador del Open ROAR
  • Scientific Electronic Library Online (SciELO)
  • Directory of Open Access Journals (DOAJ)
  • Redalyc
  • Redes sociales

  • facebook.com/repositoriokerwa
  • @Ciencia_UCR
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo depositar
  • Políticas
Contacto | Sugerencias
Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.