Development of an instrument-free and low-cost ELISA dot-blot test to detect antibodies against SARS-CoV-2
artículo original
Fecha
2023-02Autor
Apú Leitón, Navilla
Madrigal Redondo, German
Herrera Vega, María
Corrales Aguilar, Eugenia
Segura Ulate, Ismael
Metadatos
Mostrar el registro completo del ítemResumen
Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use ELISA or CLIA; however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests are a low-cost, equipment-free option, but their high price may make them less suitable for conducting seroprevalence surveys. Here, we describe a simple dual antigen ELISA dot-blot test to detect anti-SARS-CoV-2 IgG antibodies with high sensitivity (94 to 98%) and specificity (92 to 100%), compared to commercially available ELISA and CLIA options. Additionally, this ELISA dot-blot test can be completed in one hour using minimal laboratory equipment. Importantly, this immunoassay is significantly more affordable than most LFIA tests available on the global market. The dot-blot strips may be stored for up to 7 days under freezing conditions. This ELISA dot-blot test is a cost-effective option for conducting seroprevalence screenings in areas lacking ELISA or CLIA facilities, compared to LFIA tests.
External link to the item
10.1515/biol-2022-0577Colecciones
- Microbiología [1171]
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