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Properties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)

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dc.creatorAragón Ortiz, Federico
dc.creatorNanne Echandi, Clara Isabel
dc.creatorFink, Edwin
dc.date2016-02-17
dc.date.accessioned2016-05-03T15:25:38Z
dc.date.available2016-05-03T15:25:38Z
dc.descriptionThree Erythrina lectins, from E. fusca, E. globocaliz and E. costaricensis were isolated by affinity chromatography. The lectins are glycoproteins with a monomenc molecular weight of 28 000. They agglutinate human erythrocytes irrespective of blood type and the activity was inhibited by N-acetyl-galactosamine, galactose and lactose. The N-terminal amino acid sequences of the three lectins were deterrnined by automated Edman degradation of the native proteins. Comparison with the sequences of ten other legume lectins revealed extensive homology. The N-terminal amino acid for E.fusca is Val, and Ala for E. globocaliz and E. costaricensis.en-US
dc.descriptionThree Erythrina lectins, from E. fusca, E. globocaliz and E. costaricensis were isolated by affinity chromatography. The lectins are glycoproteins with a monomenc molecular weight of 28 000. They agglutinate human erythrocytes irrespective of blood type and the activity was inhibited by N-acetyl-galactosamine, galactose and lactose. The N-terminal amino acid sequences of the three lectins were deterrnined by automated Edman degradation of the native proteins. Comparison with the sequences of ten other legume lectins revealed extensive homology. The N-terminal amino acid for E.fusca is Val, and Ala for E. globocaliz and E. costaricensis.es-ES
dc.formatapplication/pdf
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/23222
dc.identifier10.15517/rbt.v42i3.23222
dc.identifier.urihttps://hdl.handle.net/10669/25680
dc.languageeng
dc.publisherUniversidad de Costa Ricaen-US
dc.rightsacceso abierto
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 42 (3) September 1994; 467-471en-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 42 (3) September 1994; 467-471es-ES
dc.sourceRevista Biología Tropical; Vol. 42 (3) September 1994; 467-471pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v42i3
dc.titleProperties and N-terminal amino acid sequences of three Erythrina lectins from Costa Rica (Leguminosae)en-US
dc.typeartículo original

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Revista de Biología Tropical