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Pastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)

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dc.coverageCRCes-ES
dc.creatorGuevara, Miguel
dc.creatorBastardo, Leandro
dc.creatorCortez, Roraysi
dc.creatorArredondo Vega, Bertha
dc.creatorRomero, Lolymar
dc.creatorGómez, Patricia
dc.date2010-12-14
dc.date.accessioned2016-05-03T15:29:08Z
dc.date.available2016-05-03T15:29:08Z
dc.descriptionRhodomonas salina (Cryptophyta) pastes as feed for Brachionus plicatilis (Rotifera). Rotifers are an important live feed for first feeding larvae of many fish species. The use of concentrated algae cells in the mass culture of the rotifer Brachionus plicatilis (Brachionidae) has opened new horizons for research on this organism. Pastes of Rhodomonas salina (Pyrenomonadaceae) obtained either by centrifugation or flocculation with chitosan were preserved, with or without vitamin C, at -20°C for four weeks and were evaluated biochemically (proteins, lipids, pigments and fatty acids contents) and subsequently, were used to feed the rotifer Brachionus plicatilis at a ratio of 25mg/L/day. Four different microalgae pastes were prepared: (1) centrifuged and preserved with vitamin C (CV), (2) centrifuged and preserved without vitamin C (C), (3) flocculated and with vitamin C (FV) and (4) flocculated without vitamin C (F). All treatments showed similar contents of proteins and total lipids with respect to control culture (a fresh culture of R. salina), with mean values of 40.0±2.32% and 12.0±1.45%, respectively. The pheophytin a/chlorophyll a ratio, a general indicator of the chemical status of microalgal concentrates, was similar (0.09-0.11) between centrifuged pastes and control culture, but was found to be higher in flocculated pastes (1.28-1.48). The fatty acid profile varied with respect to the control culture, mainly in the proportion of the essential polyunsaturated fatty acids (PUFAs): eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Total PUFAs, EPA and DHA contents were statistically similar between centrifuged pastes and control culture (PUFAs: 47%, EPA: 4% and DHA: 4.7%), whereas values obtained for flocculated pastes were significantly lower. The rotifers grew equally well when fed with centrifuged pastes or control culture (maximum density: 320rotifers/mL; instantaneous growth rate: 0.23rotifers/ day, fecundity: 1.49eggs/female and productivity: 43x103rotifers/L/day. No significant effect of vitamin C was found when used as a paste preservative. We concluded that centrifugation is an effective harvesting method, and that freezing to -20°C for four weeks (no vitamin added), may help maintain the nutritional quality of R. salina paste, similar to fresh microalgae and can be offered to Brachionus plicatilis.es-ES
dc.formatapplication/pdf
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/3415
dc.identifier10.15517/rbt.v59i4.3415
dc.identifier.urihttps://hdl.handle.net/10669/26615
dc.languagespa
dc.publisherUniversidad de Costa Ricaen-US
dc.rightsacceso abierto
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 59 (4) December 2011en-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 59 (4) December 2011es-ES
dc.sourceRevista Biología Tropical; Vol. 59 (4) December 2011pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v59i4
dc.titlePastas de Rhodomonas salina (Cryptophyta) como alimento para Brachionus plicatilis (Rotifera)es-ES
dc.typeartículo original

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Revista de Biología Tropical