Listar Microbiología por autor "4dd10ec7-e6df-49e1-b953-079d9993b8ce"
Mostrando ítems 1-20 de 24
-
Antivenomics and in vivo preclinical efficacy of six Latin American antivenoms towards south-western Colombian Bothrops asper lineage venoms
Mora Obando, Diana; Pla Ferrer, Davinia; Lomonte, Bruno; Guerrero Vargas, Jimmy; Ayerbe, Santiago; Calvete Chornet, Juan José (2021)Snakebite envenoming is an important occupational health problem, particularly in rural areas of developing countries. The timely administration of an effective antivenom remains the mainstay of snakebite management. ... -
Antivenomics: a proteomics tool for studying the immunoreactivity of antivenoms
Calvete Chornet, Juan José; Gutiérrez, José María; Sanz, Libia; Pla Ferrer, Davinia; Lomonte, Bruno (2015-03)Envenoming following snakebite is largely a neglected threat to public health in tropical and subtropical regions of Africa, Asia, Latin America and Oceania addicting some of the world’s poorest rural communities. An ... -
Assessing the preclinical efficacy of antivenoms: From the lethality neutralization assay to antivenomics
Gutiérrez, José María; Solano Trejos, María Gabriela; Pla Ferrer, Davinia; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Sanz, Libia; Lomonte, Bruno; Calvete Chornet, Juan José; León Montero, Guillermo (2013-07)The assessment of the capacity of antivenoms to neutralize the lethal activity of snake venoms is the gold standard in the preclinical analysis of antivenom efficacy, and is routinely performed by manufacturers and quality ... -
Combined venom gland cDNA sequencing and venomics of the New Guinea small-eyed snake, Micropechis ikaheka
Paiva, Owen K.; Pla Ferrer, Davinia; Wright, Christine E.; Beutler, Markus; Sanz, Libia; Gutiérrez, José María; Williams, David J.; Calvete Chornet, Juan José (2014-10-14)The venom arsenal of the New Guinea small-eyed snake, Micropechis ikaheka, was investigated by a joint cDNA sequencing and venomics approach. Twenty-seven full-length DNA sequences encoding novel venom proteins were recovered ... -
Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest
Gonçalves Machado, Larissa; Pla Ferrer, Davinia; Sanz, Libia; Jorge, Roberta Jeane B.; Leitão De Araújo, Moema; Alves, Maria Lúcia M.; Alvares, Diego Janisch; De Miranda, Joari; Nowatzki, Jenifer; de Morais Zani, Karen; Fernandes, Wilson; Tanaka Azevedo, Anita Mitico; Fernández Ulate, Julián; Zingali, Russolina B.; Gutiérrez, José María; Corrêa Netto, Carlos; Calvete Chornet, Juan José (2016-03-01)Bothrops jararaca is a slender and semi-arboreal medically relevant pit viper species endemic to tropical and subtropical forests in southern Brazil, Paraguay, and northern Argentina (Misiones). Within its geographic range, ... -
Evaluation of the preclinical efficacy of four antivenoms, distributed in sub-Saharan Africa, to neutralize the venom of the carpet viper, Echis ocellatus, from Mali, Cameroon, and Nigeria
Sánchez Céspedes, Laura Vanessa; Pla Ferrer, Davinia; Herrera Vega, María; Chippaux, Jean Philippe; Calvete Chornet, Juan José; Gutiérrez, José María (2015-11)Snakebite envenoming causes a heavy toll in sub-Saharan Africa in terms of mortality and sequelae. In the West African savannah, the viperid Echis ocellatus is responsible for the vast majority of bites. In the last decades, ... -
First crotoxin-like phospholipase A2 complex from a New World non-rattlesnake species: Nigroviriditoxin, from the arboreal Neotropical snake Bothriechis nigroviridis
Lomonte, Bruno; Mora Obando, Diana; Fernández Ulate, Julián; Sanz, Libia; Pla Ferrer, Davinia; Gutiérrez, José María; Calvete Chornet, Juan José (Toxicon vol 93:144–154, 2015-01)Bothriechis nigroviridis is an arboreal Neotropical pitviper found in Costa Rica and Panam a. A previous proteomic profiling of its venom revealed the presence of proteins with homology to the A and B subunits of ... -
Half a century of research on Bothrops asper venom variation: biological and biomedical implications
Mora Obando, Diana; Lomonte, Bruno; Pla Ferrer, Davinia; Guerrero Vargas, Jimmy; Ayerbe, Santiago; Gutiérrez, José María; Sasa Marín, Mahmood; Calvete Chornet, Juan José (2022)Snake venoms are a complex biological mixture of proteins with or without enzymatic activity, peptides, and nucleotides, among other components. It is produced in specialized secretory glands located in the maxillary region, ... -
Omics Meets Biology: Application to the Design and Preclinical Assessment of Antivenoms
Calvete Chornet, Juan José; Sanz, Libia; Pla Ferrer, Davinia; Lomonte, Bruno; Gutiérrez, José María (Toxins vol.6:3388-3405, 2014-12-15)Snakebite envenoming represents a neglected tropical disease that has a heavy public health impact worldwide, mostly affecting poor people involved in agricultural activities in Africa, Asia, Latin America and Oceania. A ... -
Preclinical assessment of a polyspecific antivenom against the venoms of Cerrophidion sasai, Porthidium nasutum and Porthidium ophryomegas: Insights from combined antivenomics and neutralization assays
Gutiérrez, José María; Tsai, Wan-Chih; Pla Ferrer, Davinia; Solano Trejos, María Gabriela; Lomonte, Bruno; Sanz, Libia; Angulo Ugalde, Yamileth; Calvete Chornet, Juan José (2013-03-15)A polyspecific antivenom is used in Central America for the treatment of envenomings by viperid snakes. This antivenom is generated in horses hyperimmunized with a mixture of venoms from Bothrops asper, Crotalus simus and ... -
Preclinical efficacy of Australian antivenoms against the venom of the small-eyed snake, Micropechis ikaheka, from Papua New Guinea: An antivenomics and neutralization study
Pla Ferrer, Davinia; Paiva, Owen K.; Sanz, Libia; Beutler, Markus; Wright, Christine E.; Calvete Chornet, Juan José; Williams, David J.; Gutiérrez, José María (2014-10-14)There is no specific antivenom for the treatment of envenoming by the small-eyed snake, Micropechis ikaheka, a dangerous fossorial species endemic to Papua New Guinea, Irian Jaya (West Papua) and neighbouring islands. This ... -
Preclinical evaluation of the efficacy of antivenoms for snakebite envenoming: State-of-the-art and challenges ahead
Solano Trejos, María Gabriela; Pla Ferrer, Davinia; Herrera Vega, María; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Sánchez Brenes, Andrés; Sanz, Libia; Lomonte, Bruno; León Montero, Guillermo; Calvete Chornet, Juan José; Gutiérrez, José María (2017-05)Animal-derived antivenoms constitute the mainstay in the therapy of snakebite envenoming. The efficacy of antivenoms to neutralize toxicity of medically-relevant snake venoms has to be demonstrated through meticulous ... -
Preclinical evaluation of three polyspecific antivenoms against the venom of Echis ocellatus: Neutralization of toxic activities and antivenomics
Calvete Chornet, Juan José; Arias Oviedo, Ana Silvia; Rodríguez, Yania; Quesada Bernat, Sarai; Sánchez Céspedes, Laura Vanessa; Chippaux, Jean Philippe; Pla Ferrer, Davinia; Gutiérrez, José María (2016-09-01)Snakebite envenoming has a heavy burden in the public health in sub-Saharan Africa. The viperid species Echis ocellatus (carpet viper or saw-scaled viper) is the medically most important snake in the savannahs of western ... -
Proteomic analysis of the mandibular glands from the Chinese crocodile lizard, Shinisaurus crocodilurus – another venomous lizard?
Calvete Chornet, Juan José; Lomonte, Bruno; Lorente, Carolina; Pla Ferrer, Davinia; Zollweg, Michael; Mebs, Dietrich (2023)Based on its phylogenetic relationship to monitor lizards (Varanidae), Gila monsters (Heloderma spp.), and the earless monitor Lanthanotus borneesis, the Chinese crocodile lizard, Shinisaurus crocodilurus, has been assigned ... -
Proteomic analysis of venom variability and ontogeny across the arboreal palm-pitvipers (genus Bothriechis)
Pla Ferrer, Davinia; Sanz, Libia; Sasa Marín, Mahmood; Acevedo, Manuel E.; Dwyer, Quetzal; Durban, Jordi; Pérez, Alicia; Rodríguez, Yania; Lomonte, Bruno; Calvete Chornet, Juan José (2017-01)Bothriechis is a genus of eleven currently recognized slender and arboreal venomous snakes, commonly called palm-pitvipers that range from southern Mexico to northern South America. Despite dietary studies suggesting that ... -
Second generation snake antivenomics: Comparing immunoaffinity and immunodepletion protocols
Pla Ferrer, Davinia; Gutiérrez, José María; Calvete Chornet, Juan José (2012-09-15)A second generation antivenomics protocol, based on affinity chromatography, was compared with a previously (first generation) immunodepletion protocol using as a proof of principle the pan-African EchiTAb-Plus-ICP® IgG ... -
Snake venomics and antivenomics of Protobothrops mucrosquamatus and Viridovipera stejnegeri from Taiwan: Keys to understand the variable immune response in horses
Villalta Arrieta, Mauren; Pla Ferrer, Davinia; Yang, Su Lin; Sanz, Libia; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Chen, Pei Yu; Herrera Vega, María; Estrada Umaña, Ricardo; Cheng, Ya Fen; Lee, Cheng Dow; Cerdas Solís, Maykel; Chiang, Jen Ron; Angulo Ugalde, Yamileth; León Montero, Guillermo; Calvete Chornet, Juan José; Gutiérrez, José María (2012-10-22)The proteomes of the venoms of the snakes Viridovipera stejnegeri and Protobothrops mucrosquamatus from Taiwan were characterized by N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation ... -
Snake venomics of Lachesis muta rhombeata and genus-wide antivenomics assessment of the paraspecific immunoreactivity of two antivenoms evidence the high compositional and immunological conservation across Lachesis
Pla Ferrer, Davinia; Sanz, Libia; Molina Sánchez, Pedro; Zorita, Virginia; Madrigal Villalobos, Marvin; Flores Díaz, Marietta; Alape Girón, Alberto; Núñez Rangel, Vitelbina; Andrés, Vicente; Gutiérrez, José María; Calvete Chornet, Juan José (2013-08-26)We report the proteomic analysis of the Atlantic bushmaster, Lachesis muta rhombeata, from Brazil. Along with previous characterization of the venom proteomes of L. stenophrys (Costa Rica), L. melanocephala (Costa Rica), ... -
Snake venomics of Micrurus alleni and Micrurus mosquitensis from the Caribbean region of Costa Rica reveals two divergent compositional patterns in New World elapids
Fernández Ulate, Julián; Vargas Vargas, Nancy; Pla Ferrer, Davinia; Sasa Marín, Mahmood; Rey Suárez, Paola; Sanz, Libia; Gutiérrez, José María; Calvete Chornet, Juan José; Lomonte, Bruno (2015-12-01)Protein composition, toxicity, and neutralization of the venoms of Micrurus alleni and Micrurus mosquitensis, two sympatric monadal coral snakes found in humid environments of the Caribbean region of Costa Rica, were ... -
Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom
Lomonte, Bruno; Pla Ferrer, Davinia; Sasa Marín, Mahmood; Tsai, Wan-Chih; Solórzano López, Alejandro; Ureña Diaz, Juan M.; Fernández Montes de Oca, María Laura; Mora Obando, Diana; Sanz, Libia; Gutiérrez, José María; Calvete Chornet, Juan José (Journal of Proteomics Volume 103, 30 May 2014, Pages 137–152, 2014-05-30)The yellow-bellied sea snake, Pelamis platura, is the most broadly distributed snake species. Despite being endowed with a highly lethal venom, a proteomic analysis of its toxin composition was unavailable. The venoms of ...