Listar Microbiología por autor "581484ad-aaa8-46b1-bc0e-f380d895640c"
Mostrando ítems 1-20 de 352
-
12-HETE is a regulator of PGE2 production via COX-2 expression induced by a snake venom group IIA phospholipase A2 in isolated peritoneal macrophages
Moreira, Vanessa; Gutiérrez, José María; Lomonte, Bruno; Ramirez Vinolo, Marco Aurélio; Curi, Rui; Lambeau, Gérard; Teixeira, Catarina de Fátima (2020)The snake venom myotoxin (MT)-III is a group IIA secreted phospholipase A2 (sPLA2) with pro-inflammatory activities. Previous studies have demonstrated that MT-III has the ability to stimulate macrophages to release ... -
A bright future for integrative venomics
Calvete Chornet, Juan José; Lomonte, Bruno (2015-10)Venomous secretions are produced by a myriad of animal species, from invertebrates to vertebrates. As a general rule, peptides and proteins represent the most abundant and functionally relevant components of these dangerous ... -
A catalytically-inactive snake venom Lys49 phospholipase A2 homolog induces expression of cyclooxygenase-2 and production of prostaglandins through selected signaling pathways in macrophages
Moreira, Vanessa; de Castro Souto, Pollyana Cristina Maggio; Ramirez Vinolo, Marco Aurélio; Lomonte, Bruno; Gutiérrez, José María; Curi, Rui; Teixeira, Catarina de Fátima (2013-05-15)The effects of a snakevenom Lys-49phospholipaseA2 (PLA2) homolog named MT-II,devoid of enzymatic activity, on the biosynthesis of prostaglandins and protein expression of cyclooxygenase-2(COX-2) and signaling pathways ... -
A constant area monolayer method to assess optimal lipid packing for lipolysis tested with several secreted phospholipases A2
Yunes Quartino, Pablo J.; Portela, Madelón; Lima, Analía; Durán, Rosario; Lomonte, Bruno; Fidelio, Gerardo Daniel (2015-10)We present an analysis of lipid monolayer hydrolysis at a constant area to assess the optimal lateral surface pressure value (Πopt) and thus, the surface packing density of the lipid, at which the activity of a given ... -
A lipidomic perspective of the action of group IIA secreted phospholipase A2 on human monocytes: lipid droplet biogenesis and activation of cytosolic phospholipase A2α
Rodríguez, Juan Pablo; Leiguez, Elbio; Guijas, Carlos; Lomonte, Bruno; Gutiérrez, José María; Teixeira, Catarina de Fátima; Balboa, María A.; Balsinde Rodríguez, Jesús (2020)Phospholipase A2s constitute a wide group of lipid-modifying enzymes which display a variety of functions in innate immune responses. In this work, we utilized mass spectrometry-based lipidomic approaches to investigate ... -
A Lys49 phospholipase A2 homologue from Bothrops asper snake venom induces proliferation, apoptosis and necrosis in a lymphoblastoid cell line
Mora Rodríguez, Rodrigo Antonio; Valverde Rojas, Berta E.; Díaz Oreiro, Cecilia; Lomonte, Bruno; Gutiérrez, José María (2005-04)Lys49 phospholipase A2 homologues are abundant in viperid snake venoms. These proteins have substitutions at the calcium-binding loop and catalytic center which render them enzymatically inactive; however, they display a ... -
A Lys49 Phospholipase A2, Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region
Giannotti, Karina Cristina; Leiguez, Elbio; Moreira, Vanessa; Galvão Nascimento, Neide; Lomonte, Bruno; Gutiérrez, José María; Lopes de Melo, Robson; Teixeira, Catarina de Fátima (2013)MT-II, a Lys49PLA2 homologue devoid of catalytic activity from B. asper venom, stimulates inflammatory events in macrophages. We investigated the ability of MT-II to induce formation of lipid droplets (LDs), key elements ... -
A Lys49-PLA2 myotoxin of Bothrops asper triggers a rapid death of macrophages that involves autocrine purinergic receptor signaling
Tonello, Fiorella; Simonato, M.; Aita, A.; Pizzo, Paola; Fernández Ulate, Julián; Lomonte, Bruno; Gutiérrez, José María; Montecucco, Cesare (Cell Death and Disease (2012) 3, e343, 2012-07-05)Lys49-PLA2 myotoxins, an important component of various viperid snake venoms, are a class of PLA2-homolog proteins deprived of catalytic activity. Similar to enzymatically active PLA2 (Asp49) and to other classes of ... -
A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina
de Roodt, Adolfo Rafael; Fernández Ulate, Julián; Solano Centeno, Daniela; Lomonte, Bruno (2018-04-27)Bothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of ... -
A new muscle damaging toxin, myotoxin II, from the venom of the snake Bothrops asper (terciopelo)
Lomonte, Bruno; Gutiérrez, José María (1989)A new muscle damaging toxin, myotoxin II, was purified from the venom of Bothrops asper by ion-exchange chromatography on CMSephadex C-25. The toxin is a dimeric, basic protein with a monomer mol.wt of 13,341, according ... -
A novel pentameric phospholipase A2 myotoxin (PophPLA2) from the venom of the pit viper Porthidium ophryomegas
Vindas González, Julio; Carrera Rengifo, Yarina; Lomonte, Bruno; Gutiérrez, José María; Calvete Chornet, Juan José; Sanz, Libia; Fernández Ulate, Julián (2018-06-07)The first toxin isolated from the venomous pit viper Porthidiumophryomegas is a basic pentameric phospholipase A2 (PophPLA2). Elucidation of its amino acid sequence showed that it belongs to the group IIA of secreted ... -
A phospholipase A2 from Bothrops asper snake venom activates neutrophils in culture: Expression of cyclooxygenase-2 and PGE2 biosynthesis
Moreira, Vanessa; Gutiérrez, José María; Bacci Amaral, Rafaela; Lomonte, Bruno; Purgatto, Eduardo; Teixeira, Catarina de Fátima (2011-02)In this study, the production of prostaglandin E2 (PGE2) and up-regulation in cyclooxygenase (COX) pathway induced by a phospholipase A2 (PLA2), myotoxin-III (MT-III), purified from Bothrops asper snake venom, in isolated ... -
A secreted phospholipase A2 induces formation of smooth muscle foam cells which trans-differentiate to macrophage-like state
Giannotti, Karina Cristina; Weinert, Sönke; Viana, Mariana Nascimento; Leiguez, Elbio; Araujo, Thaís L. S.; Laurindo, Francisco R. M.; Lomonte, Bruno; Braun Dullaeus, Rüdiger; Teixeira, Catarina de Fátima (2019)Vascular smooth muscle cells (VSMCs) loaded with lipid droplets (LDs) are markers of atherosclerosis. In this disease, inflammatory Group IIA-secreted phospholipase A2s (GIIA sPLA2s) are highly expressed in VSMCs, but ... -
A snake venom secreted phospholipase A2 induces foam cell formation depending on the activation of factors involved in lipid homeostasis
Leiguez, Elbio; do Nascimento Viana, Mariana; Hideki Matsubara, Márcio; Fernandes, Cristina Maria; Giannotti, Karina Cristina; Gutiérrez, José María; Lomonte, Bruno; Teixeira, Catarina de Fátima (2018-06)MT-III, a snake venom GIIA sPLA2, which shares structural and functional features with mammalian GIIA sPLA2s, activates macrophage defense functions including lipid droplet (LDs) formation, organelle involved in both lipid ... -
A structure-based proposal for a comprehensive myotoxic mechanism of phospholipase A2-like proteins from viperid snake venoms
Fernandes, Carlos A. H.; Borges, Rafael J.; Lomonte, Bruno; Fontes, Marcos Roberto de Mattos (Biochimica et Biophysica Acta - Proteins and Proteomics 1844(12):2265-2276, 2014-12)Envenomation via snakebites is an important public health problem in many tropical and subtropical countries that, in addition to mortality, can result in permanent sequelae as a consequence of local tissue damage, which ... -
Ability of fucoidan to prevent muscle necrosis induced by snake venom myotoxins: comparison of high- and low-molecular weight fractions
Azofeifa Chinchilla, Karol; Angulo Ugalde, Yamileth; Lomonte, Bruno (2008)Fucoidan, a natural polysaccharide extracted from brown seaweed, inhibits the myotoxic phospholipases A2 present in the venoms of crotalid snakes. This study evaluated the influence of molecular weight on the ability of ... -
Activation of cellular functions in macrophages by venom secretory Asp-49 and Lys-49 phospholipases A2
Zuliani, Juliana Pavan; Gutiérrez, José María; Casais e Silva, Luciana Lyra; Coccuzzo Sampaio, Sandra; Lomonte, Bruno; Teixeira, Catarina de Fátima (2005-10)The in vitro effects of myotoxin III (MT-III), an Asp-49 catalytically-active phospholipase A(2), and myotoxin II (MT-II), a catalytically-inactive Lys-49 variant, isolated from Bothrops asper snake venom, on phagocytosis ... -
Actividad letal de seis venenos de serpientes de importancia médica en el Ecuador
Terán Zavala, María Del Carmen; Lomonte, Bruno (2016-11)Se determinó la actividad letal de los venenos de serpientes de seis especies de vipéridos colectadas en Ecuador, a través de ratones CD-1. La especie más abundante fue Bothrops asper de la cual se analizaron adultos y ... -
La actividad proteolítica de los venenos de serpientes de Costa Rica sobre la caseína
Lomonte, Bruno; Gutiérrez, José María (Revista de Biologia Tropical 31(1), 37-40, 1983)Se establecen las condiciones óptimas para la determinación comparativa de la actividad proteolítica de los venenos de serpientes costarricenses sobre la caseína y se describe dicha actividad para 13 venenos diferentes. ... -
Activity of hemorrhagic metalloproteinase BaH-1 and myotoxin II from Bothrops asper snake venom on capillary endothelial cells in vitro
Lomonte, Bruno; Gutiérrez, José María; Borkow, Gadi; Ovadia, Michael; Tarkowski, Andrej; Hanson, Lars Ake (1994-04)In vivo, hemorrhagic toxins isolated from snake venoms cause a disorganization of the basal lamina of capillaries, with a concomitant degenerative process of endothelial cells. In this study we investigated the effects of ...