Mostrar el registro sencillo del ítem

dc.creatorLomonte, Bruno
dc.creatorMata, Eugenia
dc.creatorGutiérrez, José María
dc.date.accessioned2017-04-20T13:37:19Z
dc.date.available2017-04-20T13:37:19Z
dc.date.issued1985
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/0041010185900121es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29628
dc.description.abstractAntibodies against Bothrops asper rmyotoxin were purified from a polyvalent antivenom by affinity chromatography . These antibodies neutralized most of the myotoxic activity of crude B. asper venom, as judged by histologic evaluation of skeletal muscle and determination of plasma creatine kinase levels in mice. When tested by immunoelectrophoresis, purified antibodies formed two superimposed bands of precipitation against an homogenous by SDS-PAGE analysis) preparation of B. asper myotoxin, as well as against crude B. asper venom. Ouchterlony immunodiffusion analysis of purified antibodies showed two precipitation bands with a pattern of complete immunologic identity between samples of crude B. asper venoms from specimens collected in the Atlantic and Pacific regions of Costa Rica . In addition, these antibodies precipitated when reacted against venom of newborn B. asper specimens fromthe Pacific region, but not against venom of newborn specimens from the Atlantic region . Purified antibodies were tested by immunodiffusion against eleven different snake venoms from Costa Rica . Only the venom of B, schlegelii cross-reacted, indicating the presence in this venom of wmponents immunologically related to B. asper myotoxin. Analysis of purified antibodies to B. asper myotoxin by agarose electrophoresis and by SDS-PAGE suggests the presence of both IgG and IgM on the basis of electrophoresic position and molecular weight of the bands. Results obtained in neutralization experiments suggest that this myotoxin is a major factor in the development of local myonecrosis induced by crude B. asper venom.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-83-103]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon 23(5), 807-813 (1985)es_ES
dc.subjectmiotoxinases_ES
dc.subjectvenenoses_ES
dc.subjectserpientees_ES
dc.subjectanticuerposes_ES
dc.subjectSnake venomes_ES
dc.titleIsolation from a polyvalent antivenom of antibodies to a myotoxin in Bothrops asper snake venomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doidoi.org/10.1016/0041-0101(85)90012-1
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-83-103
dc.identifier.pmid3937296


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem