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Equine antibodies to Bothrops asper myotoxin II: isolation from polyvalent antivenom and neutralizing ability.
dc.creator | Lomonte, Bruno | |
dc.creator | Gutiérrez, José María | |
dc.creator | Carmona Morera, Elena | |
dc.creator | Rovira Rodríguez, María Elena | |
dc.date.accessioned | 2017-08-25T13:53:57Z | |
dc.date.available | 2017-08-25T13:53:57Z | |
dc.date.issued | 1990 | |
dc.identifier.citation | http://www.sciencedirect.com/science/article/pii/004101019090075I | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/73008 | |
dc.description.abstract | Equine antibodies to B. riper myotoxin II were isolated from polyvalent antivenom by affinity chromatography. Purified antibodies were among the most acidic serum immunoglobulins, migrating between the ß- and aZ-globulin regions by zone electrophoresis . This might be related to the fact that myotoxin II is a very basic antigen. At an antibody/toxin molar ratio of two or higher, myotoxic effect of myotoxin II was neutralized completely . Antibodies also neutralized myotoxic activity of crude venom in mice, reducing the effect by about 75% when a proportion of 16 mg antibodies/mg venom was tested . Despite the lack of phospholipase AZ activity of myotoxin II, antibodies were able to neutralize this enzymatic activity of myotoxin I, a previously described isoform. This finding is in agreement with the notion that myotoxin II is a phospholipase AZ-analog devoid of this enzyme activity. | es_ES |
dc.description.sponsorship | Vicerrectoria de Investigación, Universidad de Costa Rica/[No. 741-88-009]/UCR/Costa Rica | es_ES |
dc.description.sponsorship | International Foundation for Science/[No. F/1388-1]/IFS/Sweden | es_ES |
dc.description.sponsorship | Organización de las Naciones Unidas para la Educación, la Ciencia y la Cultura/[No. 1041-R]/UNESCO/ | es_ES |
dc.description.sponsorship | Costa Rican National Scientific and Technological Research Council//CONICIT/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon; Volume 28, Issue 4. 1990 | es_ES |
dc.subject | Antivenom | es_ES |
dc.subject | Antibodies | es_ES |
dc.subject | Neutralizing abilities | es_ES |
dc.title | Equine antibodies to Bothrops asper myotoxin II: isolation from polyvalent antivenom and neutralizing ability. | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/0041-0101(90)90075-I | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.pmid | 2349580 |
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Microbiología [1171]