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dc.creatorMoreira Carmona, Lisela
dc.creatorAlbertazzi Castro, Federico José
dc.creatorGarita Salazar, Laura Cristina
dc.creatorOrtiz Arias, Beatriz
dc.creatorVillalobos Muller, William
dc.date.accessioned2018-11-30T21:28:43Z
dc.date.available2018-11-30T21:28:43Z
dc.date.issued2011
dc.identifier.citationhttps://iocv.ucr.edu/proceedings/eighteen/p18.html
dc.identifier.urihttps://hdl.handle.net/10669/76196
dc.description.abstractSymptoms resembling those of Citrus variegation virus (CVV) were observed in old Palestine sweet lime trees (Citrus limettioides Tan.) used as coffee shade in the Central Valley in Costa Rica. The symptoms include leaf flecking, mosaic, malformation, and dwarfing. This disease was transmitted by grafting to “Valencia” sweet orange (C. sinensis L.), Etrog Citron (C. medica L.), and sweet lime under greenhouse conditions. Symptoms were severe in Palestine sweet lime all year long, but sweet orange and Etrog citron were asymptomatic under high temperature. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm the presence of CVV in young leaves from sweet lime trees collected from field, and other Citrus spp. grafted and maintained in the greenhouse at University of Costa Rica. Fresh tissue from trees infected with CVV obtained from the USDA (Riverside, California), was used as positive control. Furthermore, the sequence obtained (605 bp) was analyzed by Blastn algorithm which showed 97% homology with CVV RNA 3 (GenBank Accession No. AF434912). Additionally, small scale virus purification was carried out and isometric particles (26 to 32 nm) were observed under the electron microscope. To our knowledge, this is the first report of the presence of CVV in Costa Rica infecting Palestine sweet limes. Also, we remark on the potential of Palestine sweet lime as a host plant indicator in biological indexing of CVVes_ES
dc.description.sponsorshipMinisterio de Ciencia y Tecnología, Fondo Reinserción/[222-2004]/MICIT- CONICIT/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[801-A5-506]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceInternational Organization of Citrus Virologists Conference Proceedings 18th, 2011es_ES
dc.subjectCVVes_ES
dc.subjectenfermedades virus de plantases_ES
dc.subjectcítricoses_ES
dc.titleFirst Report of Citrus variegation virus in Palestine Sweet Lime, as Coffee Shade in Costa Ricaes_ES
dc.typeartículo científico
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Agronomíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.identifier.codproyecto801-A5-506


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