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dc.creatorMuñoz García, Melania Nelly
dc.creatorJiménez García, Víctor
dc.date.accessioned2019-06-28T19:49:29Z
dc.date.available2019-06-28T19:49:29Z
dc.date.issued2007-06
dc.identifier.citationhttps://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19529es_ES
dc.identifier.issn2215-2067
dc.identifier.urihttps://hdl.handle.net/10669/77952
dc.description.abstractSlipper orchids belonging to the genus Phragmipedium (subfam. Cypripedioideae) are seriously threatened and therefore listed in Appendix I of CITES. Less research has been conducted in this genus than in others belonging to the same subfamily. In this work, we evaluated development of capsules (seed pods) from Phragmipedium humboldtii, P. longifolium and P. peaarcei from the time of pollination until opening. Moreover, seed viability was tested with the tetrazolium method in each of the capsules that were subsequently used to evaluate the effect of light and two culture media (Knudson C vs. Murashige and Skoog half concentrated) on in vitro asymbiotic germination and seedling growth. 100% of the pollinated flowers developed capsules, which differentiated in terms of length and diameter among species. While the length of the capsules remained constant during development, their diameter increased during the first 6-8 weeks and then stopped. Time required for maturity and opening of the capsules also varied among species (31 weeks in P. humboldtii, 16 weeks in P. longifolium and 9.5 weeks in P. percei). Seed viability differed among species as well, averaging 34.3% in P. humboldtii, 44.7% in P. longifolium and 82.3% in P. pearcei. Furthermore, seed viability of each capsule was used to adjust the germination rate measured in each case. While very few P. humboldtii viable seeds germinated under the conditions tested (2.9%), better results were observed in the other two species (close to 40% germination). No significant effect of light/darkness regime or of culture medium was observed on germination. However, better growth of the germinated embryos was observed with the Knudson C medium and darkness conditions. Further subculture of the gro- wing plantlets under light conditions induced development of roots and allowed successful acclimatization of seedlings in the greenhouse.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[814-A6-107]/UCR/Costa Ricaes_ES
dc.language.isoeses_ES
dc.sourceLankesteriana; vol.7(1-2), pp. 310-312es_ES
dc.subjectPhragmipediumes_ES
dc.subjectOrquídeas terrestreses_ES
dc.subjectPolinizaciónes_ES
dc.subjectDesarrollo de cápsulases_ES
dc.subjectCloruro de tetrazolioes_ES
dc.subjectGerminación in vitroes_ES
dc.subject584.4 Orchidaleses_ES
dc.titleDesarrollo de cápsulas y germinación in vitro de Phragmipedium humboldtii, P. longifolium y P. perceies_ES
dc.typeartículo científicoes_ES
dc.date.updated2019-06-09T20:10:33Z
dc.identifier.doi10.15517/lank.v7i1-2.19529
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Jardín Botánico Lankester (JBL)es_ES
dc.identifier.codproyecto814-A6-107


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