Meiotic chromosome analysis in tropical orchid genus Sobralia
Fecha
2018
Tipo
contribución de congreso
Autores
Mora, José
Albertazzi, Stefano
Bolaños Villegas, Pablo Alberto
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Resumen
Tropical orchid genus Sobralia comprises terrestrial plants with elongated, cane-like stems
and large symmetrical, yet ephemeral flowers. This genus is distributed throughout Central and South
America and may hold horticultural potential. Nonetheless, little is known about the reproductive
viability of species that comprise this genus. In this project male meiocytes have been examined by
light microscopy to determine chromosome numbers, frequency of chromosome segregation defects
and the frequency of normal tetrads at the end of meiosis II. The species sampled belong to an ex situ
collection managed by the Lankester Botanical Garden of the University of Costa Rica. The species
were: S. amparoae, S. artropubescens, S. boucheri, S. bradeorum, S. carazoi, S. crispissima, S.
danjanzenii, S. fenzliana, S. geminata, S. helleri and S. rosea. Our results indicate that meiocytes from
these species have a variable diploid (2n) chromosome number of 24, 30 and 32, that segregation
defects are rare and that tetrad formation rates exceed 80%, suggesting efficient meiotic progression
and high pollen viability. Taken together our results suggest that at least in Costa Rica the populations
sampled are reproductively healthy and amenable to horticultural breeding.
Descripción
Este proyecto es una colaboración entre la Estación Experimental Agrícola Fabio Baudrit y el Jardín Botánico Lankester
Palabras clave
Sobralia, Orchid, Chromosome analysis, Meiosis, Plant breeding, 635.934 4 Orquídeas