Cariotipo de células fetales en el diagnóstico prenatal en Costa Rica
Fecha
1995
Tipo
artículo original
Autores
Castro Volio, Isabel
Escalante López, Gerardo
Mora Palma, Haroldo
Guerra Carles, Dania
Sánchez Chaves, Luis
Peña Obando, Carlos
Título de la revista
ISSN de la revista
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Editor
Resumen
De 1986 a 1992 se realizaron 182 amniocentesis entre las semanas 14 a 37 de gestación, y dos cordocentesis del tercer trimestre en 1992, para obtener el cariotipo fetal. Todos los embarazos eran de alto riesgo genético, generalmente por edad materna avanzada o por examen ultrasonográfico anormal. Se cultivaron las células fetales utilizando el sistema cerrado y la cosecha en masa. Se detectó 3.7% de cromosomopatía fetal. El tiempo promedio que se demoró el diagnostico fue de tres semanas hasta 1991 inclusive y de dos en 1992. El éxito de los cultivos varió durante los diferentes años, en 1992 se obtuvo resultados el 93% de las veces. No se detectaron errores en el diagnóstico citogénetico ni secuelas o complicaciones asociadas a la punción. Se encontró que la amniocentesis es un procedimiento seguro y confiable que permite el diagnóstico citogenético fetal y posibilita un mejor manejo obstétrico del embarazo de alto riesgo.
The results of 182 genetic amniocenteses between 14 and 37 weeks gestation, from 1986 to 1992, and of two cordocenteses in 1992, are reported. There were two main reasons for referral: maternal age 35 years and older and abnormal ultrasound assessment. Fetal cells were closed cultured and mass harvested. In 3.7% of cases fetal chromosomes were defective. Turn around time was about three weeks up to and including 1991 and two weeks in 1992, culture failure rate was 7% that year. No cytogenetic misdiagnosis and no complication or sequelae related to the amniocenteses were detected. We conclude this is a safe and reliable procedure to obtain fetal karyotypes and to improve obstetric management of high-risk pregnancies.
The results of 182 genetic amniocenteses between 14 and 37 weeks gestation, from 1986 to 1992, and of two cordocenteses in 1992, are reported. There were two main reasons for referral: maternal age 35 years and older and abnormal ultrasound assessment. Fetal cells were closed cultured and mass harvested. In 3.7% of cases fetal chromosomes were defective. Turn around time was about three weeks up to and including 1991 and two weeks in 1992, culture failure rate was 7% that year. No cytogenetic misdiagnosis and no complication or sequelae related to the amniocenteses were detected. We conclude this is a safe and reliable procedure to obtain fetal karyotypes and to improve obstetric management of high-risk pregnancies.
Descripción
artículo -- Universidad de Costa Rica. Instituto de Investigaciones en Salud, 1995
Palabras clave
prenatal diagnosis, fetal chromosomes, karyotype, pregnancy, Embarazo, Desarrollo del niño, Salud pública