The “StemDif sensor test”: a straightforward, non-invasive assay to characterize the secreted stemness and/or differentiation activities of tumor-derived cancer cell lines

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dc.creatorAbou Hammoud, Aya
dc.creatorGiraud, Julie
dc.creatorGauthereau, Xavier
dc.creatorBlanchard, Camille
dc.creatorDaburon, Sophie
dc.creatorZese, Marco
dc.creatorMolina Castro, Silvia Elena
dc.creatorDubus, Pierre
dc.creatorVaron, Chinese
dc.creatorBoeuf, Helene
dc.date.accessioned2024-10-30T19:05:53Z
dc.date.available2024-10-30T19:05:53Z
dc.date.issued2023-12-13
dc.description.abstractAbstract: Cancer stem cells are a subpopulation of tumor cells characterized by their ability to self-renew, induce tumors upon engraftment in animals and exhibit strong resistance to chemotherapy and radiotherapy. These cells exhibit numerous characteristics in common with embryonic stem cells, expressing some of their markers, typically absent in non-pathological adult differentiated cells. The aim of this study was to investigate the potential of conditioned media from cancer stem cells to modulate the fate of Leukemia Inhibitory Factor (LIF)-dependent murine embryonic stem cells (mESCs) as a way to obtain a direct readout of the secretome of cancer cells. A functional assay, “the StemDif sensor test”, was developed with two types of cancer stem cells derived from grade IV glioblastoma (adult and pediatric) or from gastric adenocarcinoma. We show that conditioned media from the selection of adult but not pediatric Glioma-Inducing Cells (GICs) maintain mESCs’ pluripotency in correlation with LIF secretion and activation of STAT3 protein. In contrast, conditioned media from gastric adenocarcinoma cells display LIF-independent stemness and differentiation activities on mESC. Our test stands out for its user friendly procedures, affordability and straightforward output, positioning it as a pioneering tool for in-depth exploration of cancer stem cell secretome characteristics.
dc.description.procedenceVicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicina
dc.description.sponsorshipCentro Nacional para la Investigación Científica/[]/CNRS/Francia
dc.description.sponsorshipRegion Aquitaine/[]//Francia
dc.description.sponsorshipInstituto Nacional de Cáncer/[PLBio 2014]/INCA/Francia
dc.description.sponsorshipLigue contre le cancer/[]//Francia
dc.description.sponsorshipInstituto Nacional de la Salud y de la Investigación Médica/[]/INSERM/Francia
dc.description.sponsorshipUniversidad de Burdeos/[]//Francia
dc.identifier.doihttps://doi.org/10.3390/biomedicines11123293
dc.identifier.issn2227-9059
dc.identifier.urihttps://hdl.handle.net/10669/100003
dc.language.isoeng
dc.rightsacceso abierto
dc.sourceBiomedicines, 11, 3293
dc.subjectSecretome
dc.subjectconditioned media
dc.subjectparacrine
dc.subjecttumor
dc.subjectLIF
dc.subjectmESCs
dc.subjectcancer stem cells
dc.titleThe “StemDif sensor test”: a straightforward, non-invasive assay to characterize the secreted stemness and/or differentiation activities of tumor-derived cancer cell lines
dc.typeartículo original

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