Genipa americana and Ageratina anisochroma, two new hosts of Candidatus Phytoplasma asteris in Costa Rica
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Authors
Villalobos Muller, William
Montero Astúa, Mauricio
Coto Morales, Teresita
Sandoval Carvajal, Izayana
Moreira Carmona, Lisela
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Abstract
We report two new plant species hosts for Candidatus Phytoplasma asteris: Genipa americana (Rubiaceae) and Ageratina anisochroma (Asteraceae). Phytoplasma infections were detected by real-time loop-mediated isothermal amplification and nestedPCR.Consensussequencesfrombothhostsshare99%identitytothe16SrI-BsubgroupusingBLAST;however,potential new subgroups are suggested due to unique RFLP patterns of the 16S rDNA F2nR2 fragment.
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Copyright © 2018, Australasian Plant Pathology Society Inc.
Keywords
Nested PCR, Real-time LAMP, Jaguatree, Guaitiltree
Citation
https://doi.org/10.1007/s13314-018-0316-3