In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation
dc.creator | Jiménez García, Víctor | |
dc.creator | Magsig Castillo, Jhamna | |
dc.creator | Tavares, Elena | |
dc.creator | Guevara Berger, Eric | |
dc.creator | Montiel Longhi, Mayra | |
dc.date.accessioned | 2019-06-24T18:47:44Z | |
dc.date.available | 2019-06-24T18:47:44Z | |
dc.date.issued | 2006 | |
dc.date.updated | 2019-06-09T19:40:25Z | |
dc.description.abstract | Guadua angustifolia Kunth was successfully propagated in vitro from axillary buds. Culture initiation, bud sprouting, shoot and plant multiplication, rooting and acclimatization, were evaluated. Best results were obtained using explants from greenhouse-cultivated plants, following a disinfection procedure that comprised the sequential use of an alkaline detergent, a mixture of the fungicide Benomyl and the bactericide Agri-mycin, followed by immersion in sodium hypochlorite (1.5% w/v) for 10 min, and culturing on Murashige and Skoog medium containing 2 ml l−1 of Plant Preservative Mixture®. Highest bud sprouting in original explants was observed when 3 mg l−1 N6-benzylaminopurine (BAP) was incorporated into the culture medium. Production of lateral shoots in in vitro growing plants increased with BAP concentration in culture medium, up to 5 mg l−1, the highest concentration assessed. After six subcultures, clumps of 8–12 axes were obtained, and their division in groups of 3–5 axes allowed multiplication of the plants. Rooting occurred in vitro spontaneously in 100% of the explants that produced lateral shoots. Successful acclimatization of well-rooted clumps of 5–6 axes was achieved in the greenhouse under mist watering in a mixture of soil, sand and rice hulls (1:1:1). | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Zootecnia | es_ES |
dc.identifier.citation | https://link.springer.com/article/10.1007%2Fs11240-006-9120-4 | |
dc.identifier.doi | 10.1007/s11240-006-9120-4 | |
dc.identifier.issn | 1573-5044 | |
dc.identifier.uri | https://hdl.handle.net/10669/77464 | |
dc.language.iso | en_US | es_ES |
dc.relation.ispartof | ||
dc.rights | acceso abierto | |
dc.source | Plant Cell, Tissue and Organ Culture; vol. 86(3), pp. 389-395 | es_ES |
dc.subject | Acclimatization | es_ES |
dc.subject | N6-benzylaminopurine (BAP) | es_ES |
dc.subject | Micropropagation | es_ES |
dc.subject | Plant growth regulators | es_ES |
dc.subject | Plant Preservative Mixture (PPM) | es_ES |
dc.subject | Rooting | es_ES |
dc.subject | 584.9 Poales (Graniinales, Pastos) | es_ES |
dc.title | In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation | es_ES |
dc.type | artículo original |