Listar Microbiología por autor "86d9e00a-76f8-4552-9d02-a03587d50166"
Mostrando ítems 1-20 de 33
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Blood flow is required for rapid endothelial cell damage induced by a snake venom hemorrhagic metalloproteinase
Gutiérrez, José María; Núñez, Javier; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra (2006-01)The effects of blood flow interruption on the ultrastructural alterations induced by a snake venom hemorrhagic metalloproteinase on skeletal muscle capillary endothelial cells were studied. Saline solution or the ... -
Bothrops asper metalloproteinase BaP1 is inhibited by alpha(2)-macroglobulin and mouse serum and does not induce systemic hemorrhage or coagulopathy
Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Kamiguti, Aura S.; Theakston, R. David G.; Gutiérrez, José María (2004-02)The ability of the P-I metalloproteinase BaP1, isolated from the venom of the snake Bothrops asper, to induce systemic bleeding, thrombocytopenia and defibrinogenation was assessed in an experimental mouse model. Intravenous ... -
Characterization of aspercetin, a platelet aggregating component from the venom of the snake Bothrops asper which induces thrombocytopenia and potentiates metalloproteinase-induced hemorrhage
Rucavado Romero, Alexandra; Soto Morera, Mónica; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Escalante Muñoz, Teresa; Gutiérrez, José María (2001-04)Thrombocytopenia occurs in a number of patients bitten by Bothrops asper, a species responsible for the majority of snakebites in Central America and southern Mexico. In this work we describe the isolation of a new ... -
Effect of the metalloproteinase inhibitor batimastat in the systemic toxicity induced by Bothrops asper snake venom: understanding the role of metalloproteinases in envenomation
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Gutiérrez, José María (2014-03-15)The peptidomimetic hydroxamate metalloproteinase inhibitor batimastat (BB-94) was assessed for its ability to neutralize the systemic effects (lethality, hemorrhage and coagulopathy) induced by the venom of Bothrops asper, ... -
Effectiveness of batimastat, a synthetic inhibitor of matrix metalloproteinases, in neutralizing local tissue damage induced by BaP1, a hemorrhagic metalloproteinase from the venom of the snake Bothrops asper
Escalante Muñoz, Teresa; Franceschi, Aida; Rucavado Romero, Alexandra; Gutiérrez, José María (2000-07-15)Batimastat (BB-94), a synthetic hydroxamate peptidomimetic matrix metalloproteinase inhibitor, was tested for its ability to inhibit proteolytic and toxic effects induced by BaP1, a 24-kDa hemorrhagic metalloproteinase ... -
Efficacy of IgG and F(ab′)2 Antivenoms to Neutralize Snake Venom-induced Local Tissue Damage as Assessed by the Proteomic Analysis of Wound Exudate
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Shannon, John D.; Ayala Castro, Carla N.; Villalta Arrieta, Mauren; Gutiérrez, José María; Fox, Jay W. (2012)Proteomic analysis of wound exudates represents a valuable tool to investigate tissue pathology and to assess the therapeutic success of various interventions. In this study, the ability of horse-derived IgG and F(ab0)2 ... -
Experimental pathology of local tissue damage induced by Bothrops asper snake venom
Gutiérrez, José María; Rucavado Romero, Alexandra; Chaves Mora, Fernando; Díaz Oreiro, Cecilia; Escalante Muñoz, Teresa (2009-12-01)Envenomations by Bothrops asper are often associated with complex and severe local pathological manifestations, including edema, blistering, dermonecrosis, myonecrosis and hemorrhage. The pathogenesis of these alterations ... -
Experimental pathophysiology of systemic alterations induced by Bothrops asper snake venom
Gutiérrez, José María; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra (2009-12-01)Moderate and severe envenomations by the snake Bothrops asper provoke systemic alterations, such as systemic bleeding, coagulopathy, hypovolemia, hemodynamic instability and shock, and acute renal failure. Systemic hemorrhage ... -
Hemorrhage Caused by Snake Venom Metalloproteinases: A Journey of Discovery and Understanding
Gutiérrez, José María; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Herrera Arias, Cristina (2016-03-26)The historical development of discoveries and conceptual frames for understanding the hemorrhagic activity induced by viperid snake venoms and by hemorrhagic metalloproteinases (SVMPs) present in these venoms is reviewed. ... -
Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage
Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Díaz Oreiro, Cecilia (2005-06-15)Zinc-dependent metalloproteinases are responsible for the hemorrhagic activity characteristic of viperid snake venoms. Snake venom metalloproteinases (SVMPs) are classified in various groups (P-I–IV), according to their ... -
High resolution analysis of snake venom metalloproteinase (SVMP) peptide bond cleavage specificity using proteome based peptide libraries and mass spectrometry
Paes Leme, Adriana F.; Escalante Muñoz, Teresa; Pereira, José G. C.; Oliveira, Ana K.; Sanchez, Eladio F.; Gutiérrez, José María; Serrano, Solange M. T.; Fox, Jay W. (2011-04-01)Both serine and metalloproteinases have been shown to play the role of toxins in the venoms of many snakes. Determination of the natural protein substrates of these toxins is an important feature in the toxinological ... -
Homogenates of skeletal muscle injected with snake venom inhibit myogenic differentiation in cell culture
Saravia Otten, Patricia; Robledo, Bárbara; Escalante Muñoz, Teresa; Bonilla, Llira; Rucavado Romero, Alexandra; Lomonte, Bruno; Hernández, Rosario; Flock, Jan-Ingmar; Gutiérrez, José María; Gastaldello, Stefano (2013-02)Introduction: Viperid snakebite envenomings are characterized by muscle necrosis and a deficient regenerative response. Methods: Homogenates from gastrocnemius muscles of mice injected with the venom of the snake Bothrops ... -
Increments in cytokines and matrix metalloproteinases in skeletal muscle after injection of tissue-damaging toxins from the venom of the snake Bothrops asper
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Teixeira, Catarina de Fátima; Fernandes, Cristina Maria; Díaz Oreiro, Cecilia; Gutiérrez, José María (2002)Envenomations by the snake Bothrops asper are characterized by prominent local tissue damage (i.e. myonecrosis), blistering, hemorrhage and edema. Various phospholipases A2 and metalloproteinases that induce local pathological ... -
Inhibition of local hemorrhage and dermonecrosis induced by Bothrops asper snake venom: effectiveness of early in situ administration of the peptidomimetic metalloproteinase inhibitor batimastat and the chelating agent CaNa2EDTA
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Franceschi, Aida; Chaves Mora, Fernando; León Montero, Guillermo; Cury, Yara; Ovadia, Michael; Gutiérrez, José María (2000-11)The effectiveness of the chelating agent CaNa2EDTA and the peptidomimetic matrix metalloproteinase inhibitor batimastat (BB-94) to inhibit local tissue damage induced by Bothrops asper snake venom was studied in mice. Both ... -
Key events in microvascular damage induced by snake venom hemorrhagic metalloproteinases
Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Fox, Jay W.; Gutiérrez, José María (2011-08-24)Hemorrhage is one of the most significant effects in envenomings induced by viperid snakebites. Damage to the microvasculature, induced by snake venom metalloproteinases (SVMPs), is the main event responsible for this ... -
Metaloproteinasas dependientes de Zinc: Protagonistas centrales en la Fisiopatología de Envenenamientos por Serpientes de la familia Viperidae
Gutiérrez, José María; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra (2016)Se presenta y discute el papel que juegan las metaloproteinasas de los venenos de serpientes (SVMPs) de la familia Viperidae en la fisiopatología de los envenenamientos inducidos por mordeduras de estos reptiles. Se hace ... -
Muscle Tissue Damage Induced by the Venom of Bothrops asper: Identification of Early and Late Pathological Events through Proteomic Analysis
Herrera Arias, Cristina; Macêdo, Jéssica Kele A.; Feoli Grant, Andrés; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Gutiérrez, José María; Fox, Jay W. (2016-04-01)The time-course of the pathological effects induced by the venom of the snake Bothrops asper in muscle tissue was investigated by a combination of histology, proteomic analysis of exudates collected in the vicinity of ... -
Novel catalytically-inactive PII metalloproteinases from a viperid snake venom with substitutions in the canonical zinc-binding motif
Camacho Umaña, Erika; Sanz, Libia; Escalante Muñoz, Teresa; Pérez, Alicia; Villalta Romero, Fabián; Lomonte, Bruno; Neves Ferreira, Ana Gisele da Costa; Feoli Grant, Andrés; Calvete Chornet, Juan José; Gutiérrez, José María; Rucavado Romero, Alexandra (2016-10-12)Snake venom metalloproteinases (SVMPs) play key biological roles in prey immobilization and digestion. The majority of these activities depend on the hydrolysis of relevant protein substrates in the tissues. Hereby, we ... -
Novel insights into capillary vessel basement membrane damage by snake venom hemorrhagic metalloproteinases: A biochemical and immunohistochemical study
Escalante Muñoz, Teresa; Shannon, John D.; Moura Da Silva, Ana M.; Gutiérrez, José María; Fox, Jay W. (2016-11-15)The hemorrhagic activity characteristic of viperid snake envenomations is due to the action of venom metalloproteinases (SVMPs) on the capillary vessel basement membrane (BM). This study compared the action of two SVMPs ... -
Proteomic analysis of Bothrops pirajai snake venom and characterization of BpirMP, a new P-I metalloproteinase
Bernardes, Carolina P.; Menaldo, Danilo L.; Camacho Umaña, Erika; Rosa, José C.; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María; Sampaio, Suely Vilela; Rosa, José C. (2013-03-27)Bothrops pirajai snake venom was analyzed by a proteomic strategy. Proteins were separated by RP-HPLC, followed by SDS-PAGE, in-gel tryptic digestion, identification by MALDI-TOF/TOF mass spectrometry, and assignment to ...